naa a22 4500 510788351 CHVBK 20180411083310.0 cr unu---uuuuu 180411e20130101xx s 000 0 eng 10.1007/s12298-012-0146-2 doi (NATIONALLICENCE)springer-10.1007/s12298-012-0146-2 Influence of plant growth regulators on callus mediated regeneration and secondary metabolites synthesis in Withania somnifera (L.) Dunal [Elektronische Daten] [Nilanjan Chakraborty, Debarupa Banerjee, Moumita Ghosh, Prakash Pradhan, Namrata Gupta, Krishnendu Acharya, Maitreyi Banerjee] Withania somnifera (L.) Dunal, is an important medicinal plant being the source of extremely important compounds like withanolides and withaferin. Influence of different plant growth regulators (PGRs) were evaluated for induction of callus, callus mediated regeneration and production of secondary metabolites in them. Explants for callusing were collected from plants grown in vitro and maximum callusing (98%) was obtained on MS medium supplemented with a combination of 2,4-dichlorophenoxy acetic acid (2,4-D) (0.5mg l-1) and kinetin (KN) (0.2mg l−1). Among different types of calli, best shoot regeneration was observed on green, compact calli produced on MS medium with a combination of 6-benzylamino purine (BAP) and indole butyric acid (IBA). MS medium supplemented with BAP (2mg l−1) showed highest frequency (98%) of shoot bud regeneration. The micro-shoots were efficiently rooted on MS media supplemented with 0.5mg l−1 IBA. Rooted plants were transferred to soil-vermi-compost (1:3; w/w) medium in greenhouse for acclimatization. Presence of withanolide A and withaferin A in calli was validated through high performance thin layer chromatography (HPTLC). It was interesting to observe that the PGRs showed significant influence on the secondary metabolites production in callus and 2,4-D having the least effect. Histological studies revealed the origin of shoot tip in the callus during regeneration. Prof. H.S. Srivastava Foundation for Science and Society, 2012 Callusing nationallicence High performance thin layer chromatography nationallicence Histology nationallicence Plant growth regulator nationallicence Shoot bud regeneration nationallicence Withaferin A nationallicence Withanolide A nationallicence PGR : Plant Growth Regulator nationallicence MS : Murashige and Skoog (1962) medium nationallicence GA3 : Gibberelic Acid A3 nationallicence 2,4-D : 2,4-dichlorophenoxy Acetic Acid nationallicence KN : kinetin nationallicence BAP : 6-benzylamino Purine nationallicence IBA : indole-3-butyric Acid nationallicence HPTLC : High Performance Thin Layer Chromatography nationallicence Chakraborty Nilanjan West Bengal State Council of Science and Technology, Bikash Bhavan, North block, 4th Floor, 700091, Salt Lake, Kolkata, India aut Banerjee Debarupa West Bengal State Council of Science and Technology, Bikash Bhavan, North block, 4th Floor, 700091, Salt Lake, Kolkata, India aut Ghosh Moumita West Bengal State Council of Science and Technology, Bikash Bhavan, North block, 4th Floor, 700091, Salt Lake, Kolkata, India aut Pradhan Prakash Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 700019, Kolkata, India aut Gupta Namrata Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 700019, Kolkata, India aut Acharya Krishnendu Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 700019, Kolkata, India aut Banerjee Maitreyi West Bengal State Council of Science and Technology, Bikash Bhavan, North block, 4th Floor, 700091, Salt Lake, Kolkata, India aut Physiology and Molecular Biology of Plants Springer-Verlag 19/1(2013-01-01), 117-125 0971-5894 19:1<117 2013 19 12298 https://doi.org/10.1007/s12298-012-0146-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-012-0146-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chakraborty Nilanjan West Bengal State Council of Science and Technology, Bikash Bhavan, North block, 4th Floor, 700091, Salt Lake, Kolkata, India aut NATIONALLICENCE 700 1- Banerjee Debarupa West Bengal State Council of Science and Technology, Bikash Bhavan, North block, 4th Floor, 700091, Salt Lake, Kolkata, India aut NATIONALLICENCE 700 1- Ghosh Moumita West Bengal State Council of Science and Technology, Bikash Bhavan, North block, 4th Floor, 700091, Salt Lake, Kolkata, India aut NATIONALLICENCE 700 1- Pradhan Prakash Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 700019, Kolkata, India aut NATIONALLICENCE 700 1- Gupta Namrata Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 700019, Kolkata, India aut NATIONALLICENCE 700 1- Acharya Krishnendu Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 700019, Kolkata, India aut NATIONALLICENCE 700 1- Banerjee Maitreyi West Bengal State Council of Science and Technology, Bikash Bhavan, North block, 4th Floor, 700091, Salt Lake, Kolkata, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer-Verlag 19/1(2013-01-01), 117-125 0971-5894 19:1<117 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer