naa a22 4500 51078836X CHVBK 20180411083310.0 cr unu---uuuuu 180411e20130101xx s 000 0 eng 10.1007/s12298-012-0137-3 doi (NATIONALLICENCE)springer-10.1007/s12298-012-0137-3 Micropropagation and non-steroidal anti-inflammatory and anti-arthritic agent boswellic acid production in callus cultures of Boswellia serrata Roxb [Elektronische Daten] [Tukaram Nikam, Ravi Ghorpade, Kirti Nitnaware, Mahendra Ahire, Vinayak Lokhande, Arvind Chopra] Micropropagation through cotyledonary and leaf node and boswellic acid production in stem callus of a woody medicinal endangered tree species Boswellia serrata Roxb. is reported. The response for shoots, roots and callus formation were varied in cotyledonary and leafy nodal explants from in vitro germinated seeds, if inoculated on Murshige and Skoog's (MS) medium fortified with cytokinins and auxins alone or together. A maximum of 8.0 ± 0.1 shoots/cotyledonary node explant and 6.9 ± 0.1 shoots/leafy node explants were produced in 91 and 88% cultures respectively on medium with 2.5μM 6-benzyladenine (BA) and 200mg l−1 polyvinylpyrrolidone (PVP). Shoots treated with 2.5μM IBA showed the highest average root number (4.5) and the highest percentage of rooting (89%). Well rooted plantlets were acclimatized and 76.5% of the plantlets showed survival upon transfer to field conditions. Randomly amplified polymorphic DNA (RAPD) analysis of the micropropagated plants compared with mother plant revealed true-to-type nature. The four major boswellic acid components in calluses raised from root, stem, cotyledon and leaf explants were analyzed using HPLC. The total content of four boswellic acid components was higher in stem callus obtained on MS with 15.0μM IAA, 5.0μM BA and 200mg l−1 PVP. The protocol reported can be used for conservation and exploitation of in vitro production of medicinally important non-steroidal anti-inflammatory metabolites of B. serrata. Prof. H.S. Srivastava Foundation for Science and Society, 2012 Anti-arthritic nationallicence Anti-inflammatory nationallicence Boswellia serrata nationallicence Boswellic acid nationallicence Burseraceae nationallicence Callus nationallicence Indian olibanum nationallicence Micropropagation nationallicence Salai guggul nationallicence Steroids nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence ABBA : Acetyl-β-boswellic acid nationallicence AKBBA : Acetyl-11-keto-β-boswellic acid nationallicence BA : 6-Benzyladenine nationallicence BBA : β-boswellic acid nationallicence IAA : Indole-3-acetic acid nationallicence KBBA : 11-keto-β-boswellic acid nationallicence Kin : Kinetin nationallicence MS : Murashige and Skoog (1962) medium nationallicence NAA : α-Naphthaleneacetic acid nationallicence PVP : Polyvinylpyrrolidone nationallicence RAPD : Randomly amplified polymorphic DNA nationallicence TDZ : Thidiazuron nationallicence HPLC : High performance liquid chromatography nationallicence Nikam Tukaram Department of Botany, University of Pune, 411 007, Pune, India aut Ghorpade Ravi Department of Botany, University of Pune, 411 007, Pune, India aut Nitnaware Kirti Department of Botany, University of Pune, 411 007, Pune, India aut Ahire Mahendra Department of Botany, University of Pune, 411 007, Pune, India aut Lokhande Vinayak Department of Botany, University of Pune, 411 007, Pune, India aut Chopra Arvind Centre for Rheumatic Diseases, 11, Hermes Elegance, 1988 Convent Street, Camp, 411 001, Pune, India aut Physiology and Molecular Biology of Plants Springer-Verlag 19/1(2013-01-01), 105-116 0971-5894 19:1<105 2013 19 12298 https://doi.org/10.1007/s12298-012-0137-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-012-0137-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nikam Tukaram Department of Botany, University of Pune, 411 007, Pune, India aut NATIONALLICENCE 700 1- Ghorpade Ravi Department of Botany, University of Pune, 411 007, Pune, India aut NATIONALLICENCE 700 1- Nitnaware Kirti Department of Botany, University of Pune, 411 007, Pune, India aut NATIONALLICENCE 700 1- Ahire Mahendra Department of Botany, University of Pune, 411 007, Pune, India aut NATIONALLICENCE 700 1- Lokhande Vinayak Department of Botany, University of Pune, 411 007, Pune, India aut NATIONALLICENCE 700 1- Chopra Arvind Centre for Rheumatic Diseases, 11, Hermes Elegance, 1988 Convent Street, Camp, 411 001, Pune, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer-Verlag 19/1(2013-01-01), 105-116 0971-5894 19:1<105 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer