naa a22 4500 510788378 CHVBK 20180411083310.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12298-013-0176-4 doi (NATIONALLICENCE)springer-10.1007/s12298-013-0176-4 Epigenetic responses to drought stress in rice ( Oryza sativa L.) [Elektronische Daten] [Gayacharan,, A. Joel] Cytosine methylation polymorphism plays a key role in gene regulation, mainly in expression of genes in crop plants. The differential expression of cytosine methylation over drought stress response was analyzed in rice using drought susceptible but agronomically superior lines IR 20 and CO 43, and drought tolerant genotypes PL and PMK 3 and their F1 hybrids. The parents and hybrids were subjected to two moisture regimes viz., one under drought condition and another under control condition. The cytosine methylation polymorphism in genomic DNA was quantified under both the conditions at the reproductive stage of the plant using the Methylation Sensitive Amplified Polymorphism (MSAP) technique devised by Xiong et al. (261:439-446, 1999). The results depicted that under drought condition, hyper-methylation was predominant in the drought susceptible genotypes while drought tolerant genotypes presented hypo-methylation behavior. While imposing drought, spikelet sterility per cent was positively correlated to percentage of methylation whereas, panicle length, number of seed per panicle, panicle weight, 100 seed weight, and yield/plant were negatively correlated indicating the role of epigenetic regulation in yield attributing traits in response to drought. Thus, methylation can be considered as an important epigenetic regulatory mechanism in rice plants to adapt drought situation. From this study, we speculate that the hyper- methylation may be an indicator of drought susceptibility and the hypo-methylation for drought tolerance and this methylation polymorphism can be effectively used in drought screening program. Prof. H.S. Srivastava Foundation for Science and Society, 2013 MSAP - Methylation Sensitive Amplification Polymorphism nationallicence AFLP - Amplified Fragment Length Polymorphism nationallicence Cytosine methylation nationallicence Epigenetics nationallicence Drought tolerance nationallicence Gayacharan, Division of Plant Genetic Resources, Indian Agricultural Research Institute, 110012, New Delhi, India aut Joel A. Department of Plant Genetic Resources, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, 641 003, Coimbatore, India aut Physiology and Molecular Biology of Plants Springer India 19/3(2013-07-01), 379-387 0971-5894 19:3<379 2013 19 12298 https://doi.org/10.1007/s12298-013-0176-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0176-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gayacharan Division of Plant Genetic Resources, Indian Agricultural Research Institute, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Joel A. Department of Plant Genetic Resources, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, 641 003, Coimbatore, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer India 19/3(2013-07-01), 379-387 0971-5894 19:3<379 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer