naa a22 4500 510788394 CHVBK 20180411083310.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12298-013-0187-1 doi (NATIONALLICENCE)springer-10.1007/s12298-013-0187-1 RETRACTED ARTICLE: Antioxidants and ROS scavenging ability in ten Darjeeling tea clones may serve as markers for selection of potentially adapted clones against abiotic stress [Elektronische Daten] [Nirjhar Dasgupta, Prosenjit Biswas, Rakesh Kumar, Narendra Kumar, Biswajit Bera, Sauren Das] Ten Darjeeling tea clones (BT15/263, RR17/144, B777, T253, B157, Sundaram, HV39, AV2, K1/1 and TTV1) were collected from the experimental garden of Darjeeling Tea Research and Development Centre, Kurseong. Total phenol, flavonoids and two antioxidating enzymes (peroxidase and superoxide dismutase) were estimated. The total phenol ranged between 241 and 28 GAE mg g−1 of leaf dry weight. The highest amount obtained in four clones, B15/263 (241.47), RR17/144 (221.2), B777 (154.54) and B157 (140.23mgg−1). Flavonoids were estimated as Catechin equivalent (CE) and ranged between 56.88 and 20.81 CE mg g−1 leaf dry weight. Higher amounts occurred in BT15/263 (56.88mgg−1), B777 (56.69) and RR17/144 (48.63). Antioxidant activities were measured following DPPH and ABTS free radicle scavenging procedures and the results were well according to total polyphenol content among the clones (in total phenols, ranges of correlation in DPPH assay were r 2  = 0.990-0.989, p ≤ 0.05; in flavonoids r 2  = 0.954, p ≤ 0.01-0.987, p ≤ 0.05). Similarly, ABTS percent scavenging results were quiet significant. The IC50 values were determined for both DPPH and ABTS assay. PAGE expressions of isoforms in two antioxidative enzymes and quantification of them also varied much among the investigated clones. The incidence of total phenols, flavonoids, PRX and SOD and ROS scavenging assay in in-situ condition, might be used as biochemical markers towards the superior adaptability against abiotic stress. In the present work, four clones (B15/263, B777, RR17/144 and B157) would be designated as comparatively better suited to the predicted abiotic stress. Prof. H.S. Srivastava Foundation for Science and Society, 2013 ABTS nationallicence Darjeeling tea nationallicence DPPH nationallicence Flavonoids nationallicence Free radicle scavenging nationallicence Peroxidase nationallicence Superoxide dismutase nationallicence Total phenols nationallicence Dasgupta Nirjhar Agricultural and Ecological Research Unit, Indian Statistical Institute, 203, B.T. Road, 700108, Kolkata, India aut Biswas Prosenjit Darjeeling Tea Research and Development Centre, A.K. Mukherjee Road, Kurseong, 734203, Darjeeling, India aut Kumar Rakesh Darjeeling Tea Research and Development Centre, A.K. Mukherjee Road, Kurseong, 734203, Darjeeling, India aut Kumar Narendra Darjeeling Tea Research and Development Centre, A.K. Mukherjee Road, Kurseong, 734203, Darjeeling, India aut Bera Biswajit Tea Board of India, Ministry of Commerce and Industry, Government of India, 14, B.T.M Sarani, 700001, Kolkata, India aut Das Sauren Agricultural and Ecological Research Unit, Indian Statistical Institute, 203, B.T. Road, 700108, Kolkata, India aut Physiology and Molecular Biology of Plants Springer India 19/3(2013-07-01), 421-433 0971-5894 19:3<421 2013 19 12298 https://doi.org/10.1007/s12298-013-0187-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0187-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dasgupta Nirjhar Agricultural and Ecological Research Unit, Indian Statistical Institute, 203, B.T. Road, 700108, Kolkata, India aut NATIONALLICENCE 700 1- Biswas Prosenjit Darjeeling Tea Research and Development Centre, A.K. Mukherjee Road, Kurseong, 734203, Darjeeling, India aut NATIONALLICENCE 700 1- Kumar Rakesh Darjeeling Tea Research and Development Centre, A.K. Mukherjee Road, Kurseong, 734203, Darjeeling, India aut NATIONALLICENCE 700 1- Kumar Narendra Darjeeling Tea Research and Development Centre, A.K. Mukherjee Road, Kurseong, 734203, Darjeeling, India aut NATIONALLICENCE 700 1- Bera Biswajit Tea Board of India, Ministry of Commerce and Industry, Government of India, 14, B.T.M Sarani, 700001, Kolkata, India aut NATIONALLICENCE 700 1- Das Sauren Agricultural and Ecological Research Unit, Indian Statistical Institute, 203, B.T. Road, 700108, Kolkata, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer India 19/3(2013-07-01), 421-433 0971-5894 19:3<421 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer