naa a22 4500 510788424 CHVBK 20180411083310.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12298-013-0182-6 doi (NATIONALLICENCE)springer-10.1007/s12298-013-0182-6 Transgenics in groundnut ( Arachis hypogaea L.) expressing cry1AcF gene for resistance to Spodoptera litura (F.) [Elektronische Daten] [G. Keshavareddy, S. Rohini, S. Ramu, S. Sundaresha, A. Kumar, P. Kumar, M. Udayakumar] Large number of primary transgenic events were generated in groundnut by an Agrobacterium mediated, in planta transformation method to assess the efficacy of cry1AcF against the Spodoptera litura. The amplification of required size fragment of 750bp with npt II primers and 901bp with cry1AcF gene primers confirmed the integration of the gene. The expression of the cry gene was ascertained by ELISA in T2 generation, and the maximum concentration of cry protein in transgenic plants reached approximately 0.82μg/g FW. Further, Southern blot analysis of ten T2 transgenic plants proved that transgene had been integrated in the genome of all the plants and Northern analysis of the same plants demonstrated the active expression of cry1AcF gene. The highest mean % larval mortalities 80.0 and 85.0 with an average mean % larval mortalities 16.25 (n = 369) and 26.0 (n = 80) were recorded in T1 and T2 generations, respectively. Segregation analysis of the selected lines in the T3 generation demonstrated homozygous nature. This clearly proved that though there is considerable improvement in average mean % larval mortality in T2 generation, the cry1AcF gene was effective against S. litura only to some extent. Prof. H.S. Srivastava Foundation for Science and Society, 2013 Groundnut nationallicence In planta transformation nationallicence cry1AcF nationallicence Spodoptera litura nationallicence Keshavareddy G. Department of Agricultural Entomology, University of Agricultural Sciences, GKVK, 560065, Bangalore, India aut Rohini S. Department of Crop Physiology, University of Agricultural Sciences, GKVK, 560065, Bangalore, India aut Ramu S. Department of Crop Physiology, University of Agricultural Sciences, GKVK, 560065, Bangalore, India aut Sundaresha S. Department of Crop Physiology, University of Agricultural Sciences, GKVK, 560065, Bangalore, India aut Kumar A. Department of Agricultural Entomology, University of Agricultural Sciences, GKVK, 560065, Bangalore, India aut Kumar P. National Research Centre on Plant Biotechnology, New Delhi, India aut Udayakumar M. Department of Crop Physiology, University of Agricultural Sciences, GKVK, 560065, Bangalore, India aut Physiology and Molecular Biology of Plants Springer India 19/3(2013-07-01), 343-352 0971-5894 19:3<343 2013 19 12298 https://doi.org/10.1007/s12298-013-0182-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0182-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Keshavareddy G. Department of Agricultural Entomology, University of Agricultural Sciences, GKVK, 560065, Bangalore, India aut NATIONALLICENCE 700 1- Rohini S. Department of Crop Physiology, University of Agricultural Sciences, GKVK, 560065, Bangalore, India aut NATIONALLICENCE 700 1- Ramu S. Department of Crop Physiology, University of Agricultural Sciences, GKVK, 560065, Bangalore, India aut NATIONALLICENCE 700 1- Sundaresha S. Department of Crop Physiology, University of Agricultural Sciences, GKVK, 560065, Bangalore, India aut NATIONALLICENCE 700 1- Kumar A. Department of Agricultural Entomology, University of Agricultural Sciences, GKVK, 560065, Bangalore, India aut NATIONALLICENCE 700 1- Kumar P. National Research Centre on Plant Biotechnology, New Delhi, India aut NATIONALLICENCE 700 1- Udayakumar M. Department of Crop Physiology, University of Agricultural Sciences, GKVK, 560065, Bangalore, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer India 19/3(2013-07-01), 343-352 0971-5894 19:3<343 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer