naa a22 4500 510788440 CHVBK 20180411083310.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12298-013-0171-9 doi (NATIONALLICENCE)springer-10.1007/s12298-013-0171-9 An efficient in vitro shoot regeneration from immature inflorescence and ex vitro rooting of Arnebia hispidissima (Lehm). DC [Elektronische Daten] [Mahendra Phulwaria, N. Shekhawat] Arnebia hispidissima, which belongs to the family Boraginaceae, is an important medicinal and dye yielding plant. The alkannin, a red dye, are root-specific secondary metabolites of A. hispidissima. Shoots were regenerated from callus derived from immature inflorescence explants obtained from field grown plants. MS medium containing 4.52μM 2, 4-D and 3.33μM BAP was found to be most effective for the proliferation of callus, induced on medium containing 4.52μM 2, 4-D. Maximum number (43.1 ± 0.25) with average length (5.2 ± 0.23) of shoots regenerated when callus was transferred to MS medium supplemented with 1.11μM BAP, 1.16μM Kin and 0.57μM IAA. About 75.5% of in vitro regenerated shoots were rooted on half-strength MS medium supplemented with 9.84μM of IBA and 200mg l−1 of activated charcoal. In comparison to in vitro, higher percent (90.2%) of shoots were rooted under ex vitro conditions when treated with IBA (0.98mM) for 5min. Plantlets rooted in vitro as well as ex vitro were acclimatized successfully under the green house conditions. Ex vitro rooted plants exhibited higher survival percentage (75%) as compared to in vitro rooted plantlets (60%). Present study may be applicable in the large-scale root-specific red dye (alkannin) production via root induction under ex vitro condition. Prof. H.S. Srivastava Foundation for Science and Society, 2013 Alkannin nationallicence Organogenesis nationallicence Secondary metabolites nationallicence 2, 4-D : 2, 4-Dichlorophenoxyacetic acid nationallicence BAP : 6-Benzylaminopurine nationallicence IAA : Indole-3 acetic acid nationallicence IBA : Indole-3 butyric acid nationallicence Kin : Kinetin nationallicence MS : Murashige and Skoog (1962) medium nationallicence NAA : Naphthalene Acetic Acid nationallicence PFD : Photon Flux Density nationallicence PGRs : Plant Growth Regulators nationallicence RH : Relative Humidity nationallicence Phulwaria Mahendra Biotechnology Unit, Department of Botany, Jai Narain Vyas University, 342001, Jodhpur, Rajasthan, India aut Shekhawat N. Biotechnology Unit, Department of Botany, Jai Narain Vyas University, 342001, Jodhpur, Rajasthan, India aut Physiology and Molecular Biology of Plants Springer India 19/3(2013-07-01), 435-441 0971-5894 19:3<435 2013 19 12298 https://doi.org/10.1007/s12298-013-0171-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0171-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Phulwaria Mahendra Biotechnology Unit, Department of Botany, Jai Narain Vyas University, 342001, Jodhpur, Rajasthan, India aut NATIONALLICENCE 700 1- Shekhawat N. Biotechnology Unit, Department of Botany, Jai Narain Vyas University, 342001, Jodhpur, Rajasthan, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer India 19/3(2013-07-01), 435-441 0971-5894 19:3<435 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer