naa a22 4500 510788467 CHVBK 20180411083311.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12298-013-0177-3 doi (NATIONALLICENCE)springer-10.1007/s12298-013-0177-3 Sequence related amplified polymorphism (SRAP) analysis for genetic diversity and micronutrient content among gene pools in mungbean [ Vigna radiata (L.) Wilczek] [Elektronische Daten] [Bharti Aneja, Neelam Yadav, Ram Yadav, Ram Kumar] Vigna radiata (L.) Wilczek, commonly called mungbean is an important pulse crop. Commercial cultivars contain low levels of iron and zinc and it is important to assess genetic variability in the available germplasm for improving micronutrient content in commercial cultivars. The present study was undertaken to study molecular diversity using Sequence-related amplified polymorphism (SRAP) among 21 Vigna radiata genotypes. Twenty nine SRAP primer combinations produced a total of 121 amplified bands which were polymorphic with an average of 4.65 bands per primer. The size of amplified bands ranged from 70bp to 3,000bp and 6 out of 29 SRAP primers were most useful in fingerprinting Vigna radiata genotypes under study. The similarity coefficients between different genotypes ranged from 0.45 to 0.96 with an average similarity value of 0.71. At an arbitrary cut-off at 60% similarity level on a dendrogram, the Vigna radiata accessions were categorized into two major clusters. ML1108 and 2KM115 were found to be genetically similar. SMH99-1A and ML776 showed high iron and zinc content while Satya was poor in iron as well as zinc content. Mapping population involving ML776 and Satya could be used for tagging gene(s) for micronutrient content. The results indicated that SRAP markers were efficient for identification of Vigna radiata genotypes and assessment of the genetic relationships among them. Prof. H.S. Srivastava Foundation for Science and Society, 2013 Mungbean nationallicence Iron and zinc content nationallicence Genetic variability nationallicence SRAP nationallicence Aneja Bharti Department of Molecular Biology and Biotechnology, Chaudhary Charan Singh Haryana Agricultural University, 125004, Hisar, India aut Yadav Neelam Department of Molecular Biology and Biotechnology, Chaudhary Charan Singh Haryana Agricultural University, 125004, Hisar, India aut Yadav Ram Department of Molecular Biology and Biotechnology, Chaudhary Charan Singh Haryana Agricultural University, 125004, Hisar, India aut Kumar Ram Department of Genetics and Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University, 125004, Hisar, India aut Physiology and Molecular Biology of Plants Springer India 19/3(2013-07-01), 399-407 0971-5894 19:3<399 2013 19 12298 https://doi.org/10.1007/s12298-013-0177-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0177-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aneja Bharti Department of Molecular Biology and Biotechnology, Chaudhary Charan Singh Haryana Agricultural University, 125004, Hisar, India aut NATIONALLICENCE 700 1- Yadav Neelam Department of Molecular Biology and Biotechnology, Chaudhary Charan Singh Haryana Agricultural University, 125004, Hisar, India aut NATIONALLICENCE 700 1- Yadav Ram Department of Molecular Biology and Biotechnology, Chaudhary Charan Singh Haryana Agricultural University, 125004, Hisar, India aut NATIONALLICENCE 700 1- Kumar Ram Department of Genetics and Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University, 125004, Hisar, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer India 19/3(2013-07-01), 399-407 0971-5894 19:3<399 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer