naa a22 4500 510788491 CHVBK 20180411083311.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12298-013-0167-5 doi (NATIONALLICENCE)springer-10.1007/s12298-013-0167-5 Copper-stress induced alterations in protein profile and antioxidant enzymes activities in the in vitro grown Withania somnifera L [Elektronische Daten] [Jyoti Rout, Shidharth Ram, Ritarani Das, Anindita Chakraborty, Mathummal Sudarshan, Santi Sahoo] Withania somnifera L. seedlings were grown in half-strength MS (Murashige and Skoog) basal medium for 4weeks and then transferred to full-strength MS liquid medium for 3weeks. The sustainable plants were subcultured in the same medium but with different concentrations (0, 25, 50, 100 and 200μM) of Cu for 7 and 14days. The growth parameters (root length, shoot length, leaf length and total number of leaves per plant) showed a declining trend in the treated plants in a concentration dependant manner. Roots and leaves were analyzed for protein profiling and antioxidant enzymes [catalase (CAT, EC 1.11.1.6), superoxide dismutase (SOD, EC 1.15.1.1) and guaiacol peroxidase (GPX, EC 1.11.1.7)]. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of crude protein extracts showed the appearance of some new proteins due to Cu treatment. In plant samples grown with 25 and 50μM of Cu, a rapid increase in antioxidant activities were noticed but at higher concentration (100 and 200μM) the activities declined. Isoforms of CAT, SOD and GPX were separated using non-denaturing polyacrylamide gel electrophoresis and concentration specific new isoforms were noticed during the study. Isoforms of the antioxidant enzymes synthesized due to Cu stress may be used as biomarkers for other species grown under metal stress. Prof. H.S. Srivastava Foundation for Science and Society, 2013 Antioxidant enzymes nationallicence Copper stress nationallicence Protein profiling nationallicence SDS-PAGE nationallicence Withania somnifera L nationallicence Rout Jyoti Biochemistry and Molecular Biology Laboratory, P.G. Department of Botany, Utkal University, Vani Vihar, 751004, Bhubaneswar, Odisha, India aut Ram Shidharth UGC-DAE Consortium of Scientific Research, Kolkata Centre, Bidhan Nagar, Salt Lake, 700098, Kolkata, India aut Das Ritarani Biochemistry and Molecular Biology Laboratory, P.G. Department of Botany, Utkal University, Vani Vihar, 751004, Bhubaneswar, Odisha, India aut Chakraborty Anindita UGC-DAE Consortium of Scientific Research, Kolkata Centre, Bidhan Nagar, Salt Lake, 700098, Kolkata, India aut Sudarshan Mathummal UGC-DAE Consortium of Scientific Research, Kolkata Centre, Bidhan Nagar, Salt Lake, 700098, Kolkata, India aut Sahoo Santi Biochemistry and Molecular Biology Laboratory, P.G. Department of Botany, Utkal University, Vani Vihar, 751004, Bhubaneswar, Odisha, India aut Physiology and Molecular Biology of Plants Springer India 19/3(2013-07-01), 353-361 0971-5894 19:3<353 2013 19 12298 https://doi.org/10.1007/s12298-013-0167-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0167-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rout Jyoti Biochemistry and Molecular Biology Laboratory, P.G. Department of Botany, Utkal University, Vani Vihar, 751004, Bhubaneswar, Odisha, India aut NATIONALLICENCE 700 1- Ram Shidharth UGC-DAE Consortium of Scientific Research, Kolkata Centre, Bidhan Nagar, Salt Lake, 700098, Kolkata, India aut NATIONALLICENCE 700 1- Das Ritarani Biochemistry and Molecular Biology Laboratory, P.G. Department of Botany, Utkal University, Vani Vihar, 751004, Bhubaneswar, Odisha, India aut NATIONALLICENCE 700 1- Chakraborty Anindita UGC-DAE Consortium of Scientific Research, Kolkata Centre, Bidhan Nagar, Salt Lake, 700098, Kolkata, India aut NATIONALLICENCE 700 1- Sudarshan Mathummal UGC-DAE Consortium of Scientific Research, Kolkata Centre, Bidhan Nagar, Salt Lake, 700098, Kolkata, India aut NATIONALLICENCE 700 1- Sahoo Santi Biochemistry and Molecular Biology Laboratory, P.G. Department of Botany, Utkal University, Vani Vihar, 751004, Bhubaneswar, Odisha, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer India 19/3(2013-07-01), 353-361 0971-5894 19:3<353 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer