naa a22 4500 510788513 CHVBK 20180411083311.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12298-013-0180-8 doi (NATIONALLICENCE)springer-10.1007/s12298-013-0180-8 Ectopic expression of Atleafy in Brassica juncea cv. Geeta for early flowering [Elektronische Daten] [Sumit Sahni, Showkat Ganie, Alka Narula, Prem Srivastava, Hari Singh] High temperature stress during pod filling severely affects the yield of Brassica juncea. Early flowering can evade the terminal heat stress and result in early maturity of the crop. In this study, a regeneration and transformation protocol has been standardized for B. juncea cv. Geeta. Hypocotyl from 5-day-old seedlings were used as explants. Of the various combinations of auxins and cytokinins tried along with Murashige and Skoog's (Physiol Plant 15:473-497, 1962) medium, MS + IAA (0.2mg/l) + BA (3mg/l) proved best for shoot regeneration with 89.9% regeneration efficiency. To induce early flowering Leafy gene from Arabidopsis thaliana was transformed using Agrobacterium mediated transformation method. After 12weeks transgenic plants showed flowering in vitro whereas their untransformed counterpart did not flower even after 16weeks. The maximum transformation frequency was 4%. Prof. H.S. Srivastava Foundation for Science and Society, 2013 Brassica juncea nationallicence Atleafy nationallicence Early flowering nationallicence Transformation nationallicence Micropropagation nationallicence Sahni Sumit Department of Biotechnology, Faculty of Science, Jamia Hamdard, Hamdard Nagar, 110062, New Delhi, India aut Ganie Showkat Department of Biotechnology, Faculty of Science, Jamia Hamdard, Hamdard Nagar, 110062, New Delhi, India aut Narula Alka Department of Biotechnology, Faculty of Science, Jamia Hamdard, Hamdard Nagar, 110062, New Delhi, India aut Srivastava Prem Department of Biotechnology, Faculty of Science, Jamia Hamdard, Hamdard Nagar, 110062, New Delhi, India aut Singh Hari Department of Science and Technology, Ministry of Science and Technology, Technology Bhawan, New Mehrauli Road, 110016, New Delhi, India aut Physiology and Molecular Biology of Plants Springer India 19/3(2013-07-01), 455-459 0971-5894 19:3<455 2013 19 12298 https://doi.org/10.1007/s12298-013-0180-8 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0180-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sahni Sumit Department of Biotechnology, Faculty of Science, Jamia Hamdard, Hamdard Nagar, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Ganie Showkat Department of Biotechnology, Faculty of Science, Jamia Hamdard, Hamdard Nagar, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Narula Alka Department of Biotechnology, Faculty of Science, Jamia Hamdard, Hamdard Nagar, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Srivastava Prem Department of Biotechnology, Faculty of Science, Jamia Hamdard, Hamdard Nagar, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Singh Hari Department of Science and Technology, Ministry of Science and Technology, Technology Bhawan, New Mehrauli Road, 110016, New Delhi, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer India 19/3(2013-07-01), 455-459 0971-5894 19:3<455 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer