naa a22 4500 510788548 CHVBK 20180411083311.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12298-013-0193-3 doi (NATIONALLICENCE)springer-10.1007/s12298-013-0193-3 In vitro propagation of two Iranian commercial pomegranates ( Punica granatum L.) cvs. ‘Malas Saveh' and ‘Yusef Khani' [Elektronische Daten] [Babak ValizadehKaji, Ahmad Ershadi, Masoud Tohidfar] An efficient in vitro propagation is described for Punica granatum L. using shoot tip and nodal explants. The influence of two basal medium, WPM and MS, and different plant growth regulators was investigated on micropropagation of the Iranian pomegranate cultivars, ‘Malas Saveh' and ‘Yousef Khani'. For proliferation stage, media supplemented with different concentrations (2.3, 4.7, 9.2 and 18.4μM) of kinetin along with 0.54μM NAA was used. WPM proved to be more efficient medium compared to MS. The best concentrations of kinetin were 4.7μM for ‘Malas Saveh' and 9.2μM for ‘Yousef Khani', resulting in the highest number of shoots per explants, shoot length and leaf number. For both cultivars, half-strength WPM medium supplemented with 5.4μM NAA was most effective for rooting of shoots. Rooted plantlets were successfully acclimatized and transferred into soil. The micropropagated plants were morphologically uniform and exhibited similar growth characteristics and vegetative morphology to the mother plants. Prof. H.S. Srivastava Foundation for Science and Society, 2013 In vitro propagation nationallicence Plant growth regulator nationallicence Pomegranate nationallicence WPM : Woody Plant Medium nationallicence MS : Murashige and Skoog nationallicence NAA : Naphthalene Acetic Acid nationallicence IBA : indole-3-butyric acid nationallicence BA : N6-Benzyladenine nationallicence BAP : 6-Benzylaminopurine nationallicence ZR : Zeatinriboside nationallicence TDZ : Thidiazuron nationallicence ValizadehKaji Babak Department of Horticultural Science, Faculty of Agriculture, Bu-Ali Sina University, Azadegan Boulevard, Hamedan, Iran aut Ershadi Ahmad Department of Horticultural Science, Faculty of Agriculture, Bu-Ali Sina University, Azadegan Boulevard, Hamedan, Iran aut Tohidfar Masoud Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, P.O. Box 31535-1897, Karaj, Iran aut Physiology and Molecular Biology of Plants Springer India 19/4(2013-10-01), 597-603 0971-5894 19:4<597 2013 19 12298 https://doi.org/10.1007/s12298-013-0193-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0193-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- ValizadehKaji Babak Department of Horticultural Science, Faculty of Agriculture, Bu-Ali Sina University, Azadegan Boulevard, Hamedan, Iran aut NATIONALLICENCE 700 1- Ershadi Ahmad Department of Horticultural Science, Faculty of Agriculture, Bu-Ali Sina University, Azadegan Boulevard, Hamedan, Iran aut NATIONALLICENCE 700 1- Tohidfar Masoud Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, P.O. Box 31535-1897, Karaj, Iran aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer India 19/4(2013-10-01), 597-603 0971-5894 19:4<597 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer