naa a22 4500 510788556 CHVBK 20180411083311.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12298-013-0199-x doi (NATIONALLICENCE)springer-10.1007/s12298-013-0199-x Micropropagation of Hedychium coronarium J. Koenig through rhizome bud [Elektronische Daten] [Pritam Mohanty, Shashikanta Behera, Swasti Swain, Durga Barik, Soumendra Naik] An optimized protocol was developed for in vitro plant regeneration of a medicinally important herb Hedychium coronarium J. Koenig using sprouted buds of rhizomes. The rhizomes with sprouted bud were inoculated on Murashige and Skoog (Physiol Plant 15:473-497, 1962) medium (MS) supplemented with either N6-benzyladenine (BA) alone (1.0-4.0mg L−1) or in combination with 0.5mg L−1 naphthalene acetic acid (NAA). Of these combinations, MS supplemented with a combination of 2.0mg L−1 BA and 0.5mg L−1 NAA was most effective. In this medium, best shoots (3.6) and roots (4.0) regeneration was observed simultaneously with an average shoot and root length of 4.7cm and 4.2cm respectively. Regeneration of shoots and roots in the same medium at the same time (One step shoot and root regeneration) reduced the time for production of in vitro plantlets and eliminates the media cost of rooting. Cent-percent (100%) success in plant establishment was observed in both gradual acclimatization process as well as when plants were directly transferred to outdoor in clay pots containing a mixture of garden soil and sand (2:1) without any sequential acclimatization stage. Prof. H.S. Srivastava Foundation for Science and Society, 2013 Hedychium coronarium nationallicence Micropropagation nationallicence Medicinal plant nationallicence Rhizome bud nationallicence BA : N6-Benzyladenine nationallicence MS : Murashige and Skoog's (1962) medium nationallicence NAA : Naphthaleneacetic acid nationallicence Mohanty Pritam Department of Botany, Ravenshaw University, 753003, Cuttack, Odisha, India aut Behera Shashikanta Department of Botany, Ravenshaw University, 753003, Cuttack, Odisha, India aut Swain Swasti Department of Botany, Ravenshaw University, 753003, Cuttack, Odisha, India aut Barik Durga Department of Botany, Ravenshaw University, 753003, Cuttack, Odisha, India aut Naik Soumendra Department of Botany, Ravenshaw University, 753003, Cuttack, Odisha, India aut Physiology and Molecular Biology of Plants Springer India 19/4(2013-10-01), 605-610 0971-5894 19:4<605 2013 19 12298 https://doi.org/10.1007/s12298-013-0199-x text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0199-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mohanty Pritam Department of Botany, Ravenshaw University, 753003, Cuttack, Odisha, India aut NATIONALLICENCE 700 1- Behera Shashikanta Department of Botany, Ravenshaw University, 753003, Cuttack, Odisha, India aut NATIONALLICENCE 700 1- Swain Swasti Department of Botany, Ravenshaw University, 753003, Cuttack, Odisha, India aut NATIONALLICENCE 700 1- Barik Durga Department of Botany, Ravenshaw University, 753003, Cuttack, Odisha, India aut NATIONALLICENCE 700 1- Naik Soumendra Department of Botany, Ravenshaw University, 753003, Cuttack, Odisha, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer India 19/4(2013-10-01), 605-610 0971-5894 19:4<605 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer