naa a22 4500 510788572 CHVBK 20180411083311.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12298-013-0194-2 doi (NATIONALLICENCE)springer-10.1007/s12298-013-0194-2 "Phloem sap analysis of Schleichera oleosa (Lour) Oken, Butea monosperma (Lam) Taub. and Ziziphus mauritiana (Lam) and hemolymph of Kerria lacca (Kerr) using HPLC and tandem mass spectrometry” [Elektronische Daten] [Amit Vashishtha, Brijesh Rathi, Sandeep Kaushik, K. Sharma, Suman Lakhanpaul] Females of lac insects especially of Kerria lacca (Kerr) secret a resin known as lac for their own protection, which has tremendous applications. Lac insect completes its lifecycle on several host taxa where it exclusively feeds on phloem sap but Schleichera oleosa (Lour.) Oken, Butea monosperma (Lam.) and Ziziphus mauritiana (Lam.) are its major hosts. Analysis of phloem sap constituents as well as hemolymph of lac insect is important because it ultimately gets converted into lac by insect intervention. Main phloem sap constituent's viz. sugars and free amino acids and hemolymph of lac insect were analyzed using HPLC and tandem mass spectrometry, respectively. The results were transformed to relative percentage of the total sugars and free amino acids analyzed in each sample for comparison among lac insect hemolymph and the phloem sap of the three different host taxa. Sucrose (58.9 ± 3.6-85.6 ± 0.9) and trehalose (62.3 ± 0.4) were the predominant sugars in phloem sap of three taxa and hemolymph of lac insect, respectively. Glutamic acid (33.1 ± 1.4-39.8 ± 1.4) was found to be main amino acid among the phloem sap of three taxa while tyrosine (61 ± 2.6) was the major amino acid in hemolymph of lac insect. The relative percentage of non-essential amino acids (60.8%-69.9%) was found to be more in all the three host taxa while essential amino acids (30.1%-35.4%) were present at a lower relative percentage. In contrast to this, the relative percentage of essential amino acids (81.9%) was observed to be higher as compared to non-essential amino acids (17.7%) in lac insect hemolymph. These results led to the detection of lac insect's endosymbionts. Moreover, this study revealed a clue regarding the importance of development of a synthetic diet for this insect so that a precise pathway of lac biosynthesis could be investigated for thorough understanding. Prof. H.S. Srivastava Foundation for Science and Society, 2013 Kerria lacca nationallicence Schleichera oleosa nationallicence Butea monosperma nationallicence Ziziphus mauritiana nationallicence Phloem sap nationallicence HPLC nationallicence Tandem mass spectrometry nationallicence Vashishtha Amit Department of Botany, University of Delhi, 110007, Delhi, India aut Rathi Brijesh Department of Chemistry, Sri Venkateswara College, University of Delhi, 110021, Delhi, India aut Kaushik Sandeep Department of Botany, University of Delhi, 110007, Delhi, India aut Sharma K. Indian Institute of Natural Resin and Gum (IINRG), 834010, Namkum, Ranchi, India aut Lakhanpaul Suman Department of Botany, University of Delhi, 110007, Delhi, India aut Physiology and Molecular Biology of Plants Springer India 19/4(2013-10-01), 537-545 0971-5894 19:4<537 2013 19 12298 https://doi.org/10.1007/s12298-013-0194-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0194-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vashishtha Amit Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Rathi Brijesh Department of Chemistry, Sri Venkateswara College, University of Delhi, 110021, Delhi, India aut NATIONALLICENCE 700 1- Kaushik Sandeep Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Sharma K. Indian Institute of Natural Resin and Gum (IINRG), 834010, Namkum, Ranchi, India aut NATIONALLICENCE 700 1- Lakhanpaul Suman Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer India 19/4(2013-10-01), 537-545 0971-5894 19:4<537 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer