naa a22 4500 510788580 CHVBK 20180411083311.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12298-013-0181-7 doi (NATIONALLICENCE)springer-10.1007/s12298-013-0181-7 Comparative assessment of ISSR, DAMD and SCoT markers for evaluation of genetic diversity and conservation of landrace chickpea ( Cicer arietinum L.) genotypes collected from north-west of Iran [Elektronische Daten] [Fatemeh Pakseresht, Reza Talebi, Ezzat Karami] Morphological traits and three molecular markers techniques: start codon targeted (SCoT), inter-simple sequence repeat (ISSR) and directly amplified minisatellite DNA (DAMD) markers were compared for fingerprinting of 40 landraces chickpea genotypes collected from different geographical locations of north-west of Iran. Variance analysis of ten measured morphological traits showed significant differences existed between genotypes. Cluster analysis based on morphological traits, divided genotypes in three distinct clusters. Average polymorphism information content (PIC) for ISSR, DAMD and SCoT markers was 0.216, 0.232 and 0.232, respectively, and this revealed that SCoT markers were more informative, followed by ISSRs marker, than other markers for the assessment of diversity amongst genotypes. Cluster analysis for three different molecular types revealed that genotypes taken for the analysis can be divided in three and four distinct clusters. Accessions from same geographical regions mostly showed more genetic similarities than those from origins far isolated apart. These results suggest that efficiency of SCOT, DAMD and ISSR markers was relatively the same in fingerprinting of genotypes but SCOT and DAMD analysis are more effective in fingerprinting of chickpea genotypes. To our knowledge, this is the first detailed report of a comparison of performance among two targeted DNA region molecular markers (SCoT and DAMD) and the ISSR technique on a set of samples of chickpea. Overall, our results indicate that SCOT, ISSR and DAMD fingerprinting could be used to detect polymorphism for genotypes of chickpea. Prof. H.S. Srivastava Foundation for Science and Society, 2013 Chickpea nationallicence DAMD nationallicence Genetic diversity nationallicence ISSR nationallicence Landrace nationallicence SCoT nationallicence DAMD : Directly amplified minisatellite DNA nationallicence ISSR : Inter simple sequence repeat nationallicence PCR : Polymerase chain reaction nationallicence PIC : Polymorphism information content nationallicence SCoT : Start codon targeted nationallicence Pakseresht Fatemeh Department of Agronomy & Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran aut Talebi Reza College of Agriculture, Sanandaj Branch, Islamic Azad University, P.O. Box: 618, Sanandaj, Iran aut Karami Ezzat College of Agriculture, Sanandaj Branch, Islamic Azad University, P.O. Box: 618, Sanandaj, Iran aut Physiology and Molecular Biology of Plants Springer India 19/4(2013-10-01), 563-574 0971-5894 19:4<563 2013 19 12298 https://doi.org/10.1007/s12298-013-0181-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0181-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pakseresht Fatemeh Department of Agronomy & Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran aut NATIONALLICENCE 700 1- Talebi Reza College of Agriculture, Sanandaj Branch, Islamic Azad University, P.O. Box: 618, Sanandaj, Iran aut NATIONALLICENCE 700 1- Karami Ezzat College of Agriculture, Sanandaj Branch, Islamic Azad University, P.O. Box: 618, Sanandaj, Iran aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer India 19/4(2013-10-01), 563-574 0971-5894 19:4<563 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer