naa a22 4500 510788610 CHVBK 20180411083311.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12298-013-0195-1 doi (NATIONALLICENCE)springer-10.1007/s12298-013-0195-1 Molecular characterization and differential expression studies of an oxidosqualene cyclase (OSC) gene of Brahmi ( Bacopa monniera ) [Elektronische Daten] [Rishi Vishwakarma, Prashant Sonawane, Somesh Singh, Uma Kumari, Ruby,, Bashir Khan] Triterpenoid saponins are the class of secondary metabolites, synthesized via isoprenoid pathway. Oxidosqualene cyclases (OSCs) catalyzes the cyclization of 2, 3-oxidosqualene to various triterpene skeletons, the first committed step in triterpenoid biosynthesis. A full-length oxidosqualene cyclase cDNA from Bacopa monniera (BmOSC) was isolated and characterized. The open reading frame (ORF) of BmOSC consists of 2,292bp, encoding 764 amino acid residues with an apparent molecular mass of 87.62kDa and theoretical pI 6.21. It contained four QxxxxxW motifs, one Asp-Cys-Thr-Ala-Glu (DCTAE) motif which is highly conserved among the triterpene synthases and another MWCYCR motif involved in the formation of triterpenoid skeletons. The deduced amino acid sequence of BmOSC shares 80.5% & 71.8% identity and 89.7% & 83.5% similarity with Olea europaea mixed amyrin synthase and Panax notoginseng dammarenediol synthase respectively. Phylogenetic analysis revealed that BmOSC is closely related with other plant OSCs. Quantitative real-time PCR (qRT-PCR) data showed that BmOSC is expressed in all tissues examined with higher expression in stem and leaves as compared to roots and floral parts. Prof. H.S. Srivastava Foundation for Science and Society, 2013 Bacopa monniera nationallicence Differential expression nationallicence Isoprenoid pathway nationallicence Oxidosqualene cyclases nationallicence Triterpenoid saponin nationallicence AMV-RT : Avian myeloblastosis virus-reverse transcriptase nationallicence OSC : Oxidosqualene cyclase nationallicence PCR : Polymerase chain reaction nationallicence RACE : Rapid amplification of cDNA ends nationallicence Vishwakarma Rishi Plant Tissue Culture Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut Sonawane Prashant Plant Tissue Culture Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut Singh Somesh Plant Tissue Culture Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut Kumari Uma Plant Tissue Culture Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut Ruby, Plant Tissue Culture Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut Khan Bashir Plant Tissue Culture Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut Physiology and Molecular Biology of Plants Springer India 19/4(2013-10-01), 547-553 0971-5894 19:4<547 2013 19 12298 https://doi.org/10.1007/s12298-013-0195-1 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0195-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vishwakarma Rishi Plant Tissue Culture Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut NATIONALLICENCE 700 1- Sonawane Prashant Plant Tissue Culture Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut NATIONALLICENCE 700 1- Singh Somesh Plant Tissue Culture Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut NATIONALLICENCE 700 1- Kumari Uma Plant Tissue Culture Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut NATIONALLICENCE 700 1- Ruby Plant Tissue Culture Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut NATIONALLICENCE 700 1- Khan Bashir Plant Tissue Culture Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, 411 008, Pune, Maharashtra, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer India 19/4(2013-10-01), 547-553 0971-5894 19:4<547 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer