naa a22 4500 510788645 CHVBK 20180411083311.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12298-013-0190-6 doi (NATIONALLICENCE)springer-10.1007/s12298-013-0190-6 Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers [Elektronische Daten] [Jagesh Tiwari, Poonam Chandel, Shruti Gupta, Jai Gopal, B. Singh, Vinay Bhardwaj] The genetic stability of in vitro propagated potato microtubers was assessed using random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. Microtubers were developed through in vitro from potato microplants using standardized protocols. The microtubers were conserved for 1year under three different culture media and consequently microplants were regenerated for the DNA analyses. During the study, a total of 38 (10 RAPD, 11 ISSR, 12 SSR and 5 AFLP) primers produced a total of 407 (58 RAPD, 56 ISSR, 96 SSR and 197 AFLP) clear, distinct and reproducible amplicons. Cluster analysis revealed 100% genetic similarity among the mother plant and its derivatives within the clusters by SSR, ISSR and RAPD analyses, whereas AFLP analysis revealed from 85 to 100% genetic similarity. Dendrogram analysis based on the Jaccard's coefficient classified the genotypes into five clusters (I-V), each cluster consisting of mother plant and its derivatives. Principal component analysis (PCA) also plotted mother plant and its genotypes of each cluster together. Based on our results, it is concluded that AFLP is the best method followed by SSR, ISSR and RAPD to detect genetic stability of in vitro conserved potato microtubers. The in vitro conservation medium (T2) is a safe method for conservation of potato microtubers to produce true-to-type plans. Prof. H.S. Srivastava Foundation for Science and Society, 2013 In vitro conservation nationallicence Genetic stability nationallicence Microtubers nationallicence Molecular markers nationallicence Potato nationallicence Tiwari Jagesh Central Potato Research Institute, 171 001, Shimla, Himachal Pradesh, India aut Chandel Poonam Central Potato Research Institute, 171 001, Shimla, Himachal Pradesh, India aut Gupta Shruti Central Potato Research Institute, 171 001, Shimla, Himachal Pradesh, India aut Gopal Jai Central Potato Research Institute, 171 001, Shimla, Himachal Pradesh, India aut Singh B. Central Potato Research Institute, 171 001, Shimla, Himachal Pradesh, India aut Bhardwaj Vinay Central Potato Research Institute, 171 001, Shimla, Himachal Pradesh, India aut Physiology and Molecular Biology of Plants Springer India 19/4(2013-10-01), 587-595 0971-5894 19:4<587 2013 19 12298 https://doi.org/10.1007/s12298-013-0190-6 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0190-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tiwari Jagesh Central Potato Research Institute, 171 001, Shimla, Himachal Pradesh, India aut NATIONALLICENCE 700 1- Chandel Poonam Central Potato Research Institute, 171 001, Shimla, Himachal Pradesh, India aut NATIONALLICENCE 700 1- Gupta Shruti Central Potato Research Institute, 171 001, Shimla, Himachal Pradesh, India aut NATIONALLICENCE 700 1- Gopal Jai Central Potato Research Institute, 171 001, Shimla, Himachal Pradesh, India aut NATIONALLICENCE 700 1- Singh B. Central Potato Research Institute, 171 001, Shimla, Himachal Pradesh, India aut NATIONALLICENCE 700 1- Bhardwaj Vinay Central Potato Research Institute, 171 001, Shimla, Himachal Pradesh, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer India 19/4(2013-10-01), 587-595 0971-5894 19:4<587 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer