naa a22 4500 510788661 CHVBK 20180411083311.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12298-013-0196-0 doi (NATIONALLICENCE)springer-10.1007/s12298-013-0196-0 Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean ( Phaseolus vulgaris L.) exposed to heavy metals [Elektronische Daten] [Darinka Gjorgieva, Tatjana Kadifkova Panovska, Tatjana Ruskovska, Katerina Bačeva, Trajče Stafilov] The present study aimed to analyze the biological effects induced by bioaccumulation of metals in common bean (Phaseolus vulgaris L.). Effects of mineral nutrient imbalance, total antioxidants level and DNA damage induced by accumulation of heavy metals, were investigated in bean seedlings treated with two selected metal concentrations for 7days. Metal content is analyzed by inductively coupled plasma - atomic emission spectrometer (ICP-AES), for total antioxidants level assessment the Ferric-Reducing Antioxidant Power (FRAP) assay is used and Random Amplified Polymorphic DNA (RAPD) method was applied for investigation of DNA damages. The increasing metal concentration in the treatment medium changed synchronously metal content in samples, and decreased total antioxidant activity in all samples with exception only for samples treated with Ni and Cd. The obtained "DNA fingerprints” demonstrated that the increasing metal concentrations induced changes in RAPD profiles (disappearance and/or appearance of bands in comparison with untreated control samples). The highest number of missing bands was observed in samples treated with zinc (total 4 bands) and nickel (total 4 bands) at both concentrations. These results suggested that mineral nutrient imbalance is involved in changes of antioxidant levels and DNA damages of the seedlings, which may help to understand the mechanism of metal toxicity in plants. Prof. H.S. Srivastava Foundation for Science and Society, 2013 Genotoxicity nationallicence RAPD nationallicence FRAP nationallicence Heavy metals nationallicence Phaseolus vulgaris nationallicence Gjorgieva Darinka Faculty of Medical Sciences, Goce Delčev University, Krste Misirkov str. bb, POB 201, 2000, Štip, Macedonia aut Kadifkova Panovska Tatjana Faculty of Pharmacy, Ss. Cyril and Methodius University, Skopje, Macedonia aut Ruskovska Tatjana Faculty of Medical Sciences, Goce Delčev University, Krste Misirkov str. bb, POB 201, 2000, Štip, Macedonia aut Bačeva Katerina Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, Skopje, Macedonia aut Stafilov Trajče Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, Skopje, Macedonia aut Physiology and Molecular Biology of Plants Springer India 19/4(2013-10-01), 499-507 0971-5894 19:4<499 2013 19 12298 https://doi.org/10.1007/s12298-013-0196-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0196-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gjorgieva Darinka Faculty of Medical Sciences, Goce Delčev University, Krste Misirkov str. bb, POB 201, 2000, Štip, Macedonia aut NATIONALLICENCE 700 1- Kadifkova Panovska Tatjana Faculty of Pharmacy, Ss. Cyril and Methodius University, Skopje, Macedonia aut NATIONALLICENCE 700 1- Ruskovska Tatjana Faculty of Medical Sciences, Goce Delčev University, Krste Misirkov str. bb, POB 201, 2000, Štip, Macedonia aut NATIONALLICENCE 700 1- Bačeva Katerina Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, Skopje, Macedonia aut NATIONALLICENCE 700 1- Stafilov Trajče Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, Skopje, Macedonia aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer India 19/4(2013-10-01), 499-507 0971-5894 19:4<499 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer