naa a22 4500 510788688 CHVBK 20180411083311.0 cr unu---uuuuu 180411e20131001xx s 000 0 eng 10.1007/s12298-013-0191-5 doi (NATIONALLICENCE)springer-10.1007/s12298-013-0191-5 Isolation of a fruit ripening-related tonoplast aquaporin (GjTIP) gene from Gardenia jasminoides [Elektronische Daten] [Lan Gao, Yi-jun Guo] Aquaporins are membrane water channels that play critical roles in controlling the water content of cells and tissues. In this work, a full-length cDNA encoding putative aquaporins was isolated from Gardenia jasminoides fruit cDNA library. The GjTIP cDNA is 1188bp, contains a predicted 774bp open reading frame that encodes 257 amino acids. A phylogenetic analysis conducted with previously characterized aquaporins from other plant species indicates that the cDNA encode putative tonoplast aquaporins (TIPs), and proposed that GjTIP has a tendency to be a mixed function aquaporin similar to the TIP1s from Arabidopsis and Gossypium raimondii. A typical "hourglasses” three-dimensional model of GjTIP was built. The expression of the GjTIP transcripts at fruits during maturation was conducted by RT-PCR analysis. The data revealed that the transcript levels of GjTIP have increased during fruit maturation. Prof. H.S. Srivastava Foundation for Science and Society, 2013 Gardenia jasminoides nationallicence Tonoplast aquaporin nationallicence Expression nationallicence Fruits nationallicence Gao Lan School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, 510006, Guangzhou, Peoples Republic of China aut Guo Yi-jun School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, 510006, Guangzhou, Peoples Republic of China aut Physiology and Molecular Biology of Plants Springer India 19/4(2013-10-01), 555-561 0971-5894 19:4<555 2013 19 12298 https://doi.org/10.1007/s12298-013-0191-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0191-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gao Lan School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, 510006, Guangzhou, Peoples Republic of China aut NATIONALLICENCE 700 1- Guo Yi-jun School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, 510006, Guangzhou, Peoples Republic of China aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer India 19/4(2013-10-01), 555-561 0971-5894 19:4<555 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer