naa a22 4500 510788726 CHVBK 20180411083311.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s12298-012-0159-x doi (NATIONALLICENCE)springer-10.1007/s12298-012-0159-x Bhattacharjee Soumen Post Graduate Department of Botany, Hooghly Mohsin College (West Bengal Education Service), 712101, Chinsurah, West Bengal, India aut Heat and chilling induced disruption of redox homeostasis and its regulation by hydrogen peroxide in germinating rice seeds ( Oryza sativa L., Cultivar Ratna) [Elektronische Daten] [Soumen Bhattacharjee] Extremes of temperature (both heat and chilling) during early inbibitional phase of germination caused disruption of redox-homeostasis by increasing accumulation of reactive oxygen species (superoxide and hydrogen peroxide) and significant reduction of antioxidative defense (assessed in terms of total thiol content and activities of superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase) in germinating tissues of rice (Oryza sativa L., cultivar Ratna). Imbibitional heat and chilling stress also induced oxidative damage to newly assembled membrane system by aggravating membrane lipid peroxidation and protein oxidation [measured in terms of thiobarbituric acid reactive substances (TBARS), free carbonyl content (C = O groups) and membrane protein thiol level (MPTL)]. Treatment with standardized low titer hydrogen peroxide during early imbibitional phase of germination caused significant reversal in oxidative damages to the newly assembled membrane system imposed by heat and chilling stress [evident from the data of TBARS, C = O, MPTL, ROS accumulation, membrane permeability status, membrane injury index and oxidative stress index] in seedlings of experimental rice cultivar. Imbibitional H2O2 pretreatment also caused up-regulation of antioxidative defense (activities of superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase and total thiol content) in the heat and chilling stress-raised rice seedlings. When the parameters of early growth performances were assessed (in terms of relative growth index, biomass accumulation, relative germination performance, mean daily germination, T50 value), it clearly exhibited significant improvement of early growth performances of the experimental rice cultivar. The result proposes that an ‘inductive pulse' of H2O2 is required to switch on some stress acclimatory metabolism through which plant restores redox homeostasis and prevents or repairs oxidative damages to newly assembled membrane system caused by unfavorable environmental cues during early germination to the rice cultivar Ratna. The importance of mitigating oxidative damages to membrane lipid and protein necessary for post-germinative growth under extremes of temperature is also suggested. Prof. H.S. Srivastava Foundation for Science and Society, 2013 Temperature stress nationallicence Oxidative membrane damage nationallicence Hydrogen peroxide nationallicence Redox regulation nationallicence Antioxidative defense nationallicence Protein oxidation nationallicence Lipid peroxidation nationallicence Rice nationallicence Physiology and Molecular Biology of Plants Springer-Verlag 19/2(2013-04-01), 199-207 0971-5894 19:2<199 2013 19 12298 https://doi.org/10.1007/s12298-012-0159-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-012-0159-x text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Bhattacharjee Soumen Post Graduate Department of Botany, Hooghly Mohsin College (West Bengal Education Service), 712101, Chinsurah, West Bengal, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer-Verlag 19/2(2013-04-01), 199-207 0971-5894 19:2<199 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer