naa a22 4500 510788769 CHVBK 20180411083312.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s12298-013-0166-6 doi (NATIONALLICENCE)springer-10.1007/s12298-013-0166-6 RAPD, SCAR and conserved 18S rDNA markers for a red-listed and endemic medicinal plant species, Knema andamanica ( Myristicaceae ) [Elektronische Daten] [T. Sheeja, P. Anju, R. Shalini, S. Siju, K. Dhanya, B. Krishnamoorthy] Knema andamanica is a red-listed endemic medicinal species of Myristicaceae restricted to Andaman and Nicobar (A&N) Islands, India. This species is used in tribal medicines and has immense bioprospective potential. With a view to generate suitable genomic markers for classification and identification, we have generated RAPD, SCAR and conserved 18S rDNA markers from K. andamanica. A unique 585bp fragment, that distinguished it from seven other related species of Myristicaceae was first amplified using the random primer OPE 06 and converted to SCAR marker (GenBank accession # JN228256). The conserved sequences of 18S rDNA loci from K. andamanica were also amplified and sequenced (GenBank accession #JN228265). The sequence revealed deviations including 18 variable regions and 15 indels that were unique to K. andamanica. These markers can help in definite identification of K. andamanica even at the juvenile stages. Prof. H.S. Srivastava Foundation for Science and Society, 2013 Knema andamanica nationallicence RAPD nationallicence SCAR nationallicence 18S rDNA and germplasm characterization nationallicence Genetic differentiation nationallicence SCAR : Sequence Characterised Amplified Region nationallicence ISSR : Inter Simple Sequence Repeats nationallicence AFLP : Amplified Fragment Length Polymorphism nationallicence SSR : Simple Sequence Repeats nationallicence rDNA : ribosomal DNA nationallicence NCBI : National Center for Biotechnology Information nationallicence RAPD : Random Amplification of Polymorphic DNA nationallicence SNP : Single Nucleotide Polymorphism nationallicence BLAST : Basic Local Alignment Search Tool nationallicence Sheeja T. Division of Crop Improvement and Biotechnology, Indian Institute of Spices Research, Marikunnu PO, 673012, Calicut, Kerala, India aut Anju P. Division of Crop Improvement and Biotechnology, Indian Institute of Spices Research, Marikunnu PO, 673012, Calicut, Kerala, India aut Shalini R. Division of Crop Improvement and Biotechnology, Indian Institute of Spices Research, Marikunnu PO, 673012, Calicut, Kerala, India aut Siju S. Division of Crop Improvement and Biotechnology, Indian Institute of Spices Research, Marikunnu PO, 673012, Calicut, Kerala, India aut Dhanya K. Division of Crop Improvement and Biotechnology, Indian Institute of Spices Research, Marikunnu PO, 673012, Calicut, Kerala, India aut Krishnamoorthy B. Division of Crop Improvement and Biotechnology, Indian Institute of Spices Research, Marikunnu PO, 673012, Calicut, Kerala, India aut Physiology and Molecular Biology of Plants Springer-Verlag 19/2(2013-04-01), 245-250 0971-5894 19:2<245 2013 19 12298 https://doi.org/10.1007/s12298-013-0166-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0166-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sheeja T. Division of Crop Improvement and Biotechnology, Indian Institute of Spices Research, Marikunnu PO, 673012, Calicut, Kerala, India aut NATIONALLICENCE 700 1- Anju P. Division of Crop Improvement and Biotechnology, Indian Institute of Spices Research, Marikunnu PO, 673012, Calicut, Kerala, India aut NATIONALLICENCE 700 1- Shalini R. Division of Crop Improvement and Biotechnology, Indian Institute of Spices Research, Marikunnu PO, 673012, Calicut, Kerala, India aut NATIONALLICENCE 700 1- Siju S. Division of Crop Improvement and Biotechnology, Indian Institute of Spices Research, Marikunnu PO, 673012, Calicut, Kerala, India aut NATIONALLICENCE 700 1- Dhanya K. Division of Crop Improvement and Biotechnology, Indian Institute of Spices Research, Marikunnu PO, 673012, Calicut, Kerala, India aut NATIONALLICENCE 700 1- Krishnamoorthy B. Division of Crop Improvement and Biotechnology, Indian Institute of Spices Research, Marikunnu PO, 673012, Calicut, Kerala, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer-Verlag 19/2(2013-04-01), 245-250 0971-5894 19:2<245 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer