naa a22 4500 510788793 CHVBK 20180411083312.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s12298-012-0158-y doi (NATIONALLICENCE)springer-10.1007/s12298-012-0158-y In vitro regeneration in Sarcostemma acidum (Roxb.) -an important medicinal plant of semi-arid ecosystem of Rajasthan, India [Elektronische Daten] [Mahender Rathore, Narpat Shekhawat] An efficient regeneration protocol for Sarcostemma acidum - an important medicinal plant has been established. Callus initiated from nodal explant on MS medium with 2.0mg L−1 of NAA + additives. Callus initiated was subcultured on MS medium containing various concentrations of NAA or 2,4-D. Out of these combinations, MS medium +1.0mg L−1 of NAA + additives was found to be effective for the multiplication of callus. Subculture was done after an interval of 20-22days. For differentiation of callus BAP or Kinetin alone was found to be less effective. Maximum frequency of shoot regeneration recorded on MS medium +1.0mg L−1 of BAP + 0.5mg L−1 of Kinetin and 0.1mg L−1 of NAA + additives. The in vitro differentiated shoots were excised and inoculated on 1/4 strength MS medium +2.0mg L−1 of IBA + 0.02% activated charcoal for in vitro rooting. Maximum response (90 %) was recorded on this medium. In vitro differentiated shoots were inoculated on autoclaved soilrite® after treatment with root inducing auxins. Ex vitro rooting in this plant species has been reported for the first time. Eighty five percent of the shoots rooted under ex vitro conditions. Both in vitro and ex vitro rooted plantlets were hardened in a green house. Prof. H.S. Srivastava Foundation for Science and Society, 2013 Adenine sulphate nationallicence Ex vitro nationallicence Hardening nationallicence In vitro nationallicence Micropropagation nationallicence Sarcostemma acidum nationallicence BAP : 6-Benzylaminopurine nationallicence IAA : Indole-3 acetic acid nationallicence IBA : Indole-3 butyric acid nationallicence Kin : Kinetin nationallicence MS : Murashige and Skoog medium nationallicence NAA : α-Naphthalene acetic acid nationallicence PGR : Plant growth regulator nationallicence RH : Relative humidity nationallicence SFP : Spectral flux photon nationallicence Rathore Mahender Biotechnology Unit, Jai Narain Vyas University, 342033, Jodhpur, Rajasthan, India aut Shekhawat Narpat Biotechnology Unit, Jai Narain Vyas University, 342033, Jodhpur, Rajasthan, India aut Physiology and Molecular Biology of Plants Springer-Verlag 19/2(2013-04-01), 269-275 0971-5894 19:2<269 2013 19 12298 https://doi.org/10.1007/s12298-012-0158-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-012-0158-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rathore Mahender Biotechnology Unit, Jai Narain Vyas University, 342033, Jodhpur, Rajasthan, India aut NATIONALLICENCE 700 1- Shekhawat Narpat Biotechnology Unit, Jai Narain Vyas University, 342033, Jodhpur, Rajasthan, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer-Verlag 19/2(2013-04-01), 269-275 0971-5894 19:2<269 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer