naa a22 4500 510788815 CHVBK 20180411083312.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s12298-012-0154-2 doi (NATIONALLICENCE)springer-10.1007/s12298-012-0154-2 Cloning and characterization of cold, salt and drought inducible C-repeat binding factor gene from a highly cold adapted ecotype of Lepidium latifolium L [Elektronische Daten] [M. Akhtar, A. Jaiswal, J. Jaiswal, M. Qureshi, M. Tufchi, N. Singh] The dehydration-responsive element-binding (DREB) protein/C-Repeat Binding Factors (CBFs) belongs to APETALA2 (AP2) family transcription factors that binds to DRE/CRT cis-element in cold-responsive (COR) genes and induce COR genes. CBFs have been isolated and characterized from evolutionarily diverse plant species. CBF pathway is conserved by CBF regulon and the size or the number and kind of target genes vary among freezing sensitive and tolerant plants. Hence, cloning of CBFs from highly freezing tolerant plants such as Lepidium latifolium L. will be useful in understanding the freezing tolerance of this species. In this study, LlCBF, a CBF1 family gene from L. Latifolium L., was cloned using RT-PCR and RACE-PCR. The full length mRNA of LlCBF is 948bp with an open reading frame of 642bp, encoding a protein of 213 amino acids with a molecular weight of 23.92kDa and a theoretical isoelectric point of 4.80. Amino acid sequence analysis showed that LlCBF has an AP2 DNA binding domain, a potential CBF type nuclear localization signal (NLS) and C-terminal acidic domain. Semi-quantitative RT-PCR analysis of LlCBF revealed that this gene is up-regulated by high salt, dehydration and low temperature stresses. The investigation is therefore successful in cloning of a gene having strong homology with CBF transcription factors and responsive to low temperature, high salt and dehydration conditions. Prof. H.S. Srivastava Foundation for Science and Society, 2012 Lepidium latifolium L nationallicence Cold nationallicence Salt and drought inducible nationallicence LlCBF transcription factor nationallicence AP2/EREBP : Apetala2/Ethylene responsive element binding protein nationallicence NLS : Nuclear localization signal nationallicence DREB/CBF : Dehydration response element binding/C-repeat binding factor nationallicence ICE1 : Inducer of CBF expression 1 nationallicence COR : Cold-regulated nationallicence HOS1 : High expression of osmotically responsive genes 1 nationallicence PCR : Polymerase chain reaction nationallicence RT-PCR : Reverse transcriptase PCR nationallicence RACE : Rapid amplification of cDNA ends nationallicence ORF : Open reading frame nationallicence EST : Expressed sequence tag nationallicence Akhtar M. Department of Genetics and Plant Breeding, College of Agriculture, G. B. Pant University of Agriculture & Technology, 263145, Pantnagar, Udhamsingh Nagar, Uttarakhand, India aut Jaiswal A. Department of Genetics and Plant Breeding, College of Agriculture, G. B. Pant University of Agriculture & Technology, 263145, Pantnagar, Udhamsingh Nagar, Uttarakhand, India aut Jaiswal J. Department of Genetics and Plant Breeding, College of Agriculture, G. B. Pant University of Agriculture & Technology, 263145, Pantnagar, Udhamsingh Nagar, Uttarakhand, India aut Qureshi M. Department of Biotechnology, Faculty of Natural Sciences, Jamia Millia Islamia, New Delhi, India aut Tufchi M. Department of Molecular Biology and Biotechnology, G. B. Pant University of Agriculture & Technology, 263145, Pantnagar, Udhamsingh Nagar, Uttarakhand, India aut Singh N. Department of Genetics and Plant Breeding, College of Agriculture, G. B. Pant University of Agriculture & Technology, 263145, Pantnagar, Udhamsingh Nagar, Uttarakhand, India aut Physiology and Molecular Biology of Plants Springer-Verlag 19/2(2013-04-01), 221-230 0971-5894 19:2<221 2013 19 12298 https://doi.org/10.1007/s12298-012-0154-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-012-0154-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Akhtar M. Department of Genetics and Plant Breeding, College of Agriculture, G. B. Pant University of Agriculture & Technology, 263145, Pantnagar, Udhamsingh Nagar, Uttarakhand, India aut NATIONALLICENCE 700 1- Jaiswal A. Department of Genetics and Plant Breeding, College of Agriculture, G. B. Pant University of Agriculture & Technology, 263145, Pantnagar, Udhamsingh Nagar, Uttarakhand, India aut NATIONALLICENCE 700 1- Jaiswal J. Department of Genetics and Plant Breeding, College of Agriculture, G. B. Pant University of Agriculture & Technology, 263145, Pantnagar, Udhamsingh Nagar, Uttarakhand, India aut NATIONALLICENCE 700 1- Qureshi M. Department of Biotechnology, Faculty of Natural Sciences, Jamia Millia Islamia, New Delhi, India aut NATIONALLICENCE 700 1- Tufchi M. Department of Molecular Biology and Biotechnology, G. B. Pant University of Agriculture & Technology, 263145, Pantnagar, Udhamsingh Nagar, Uttarakhand, India aut NATIONALLICENCE 700 1- Singh N. Department of Genetics and Plant Breeding, College of Agriculture, G. B. Pant University of Agriculture & Technology, 263145, Pantnagar, Udhamsingh Nagar, Uttarakhand, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer-Verlag 19/2(2013-04-01), 221-230 0971-5894 19:2<221 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer