naa a22 4500 510788823 CHVBK 20180411083312.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s12298-012-0152-4 doi (NATIONALLICENCE)springer-10.1007/s12298-012-0152-4 In vitro clonal propagation and genetic fidelity of the regenerants of Spilanthes calva DC. using RAPD and ISSR marker [Elektronische Daten] [Mohd. Razaq, Monika Heikrujam, Siva Chetri, Veena Agrawal] An efficient in vitro protocol has been established for clonal propagation of elite plant of Spilanthes calva DC., an important source of spilanthol, an antimalarial larvicidal compound. Nodal explants excised from field grown plant of S. calva DC. when reared on Murashige and Skoog's medium augmented with different cytokinins, viz. N6-Benzyladenine (BA), N6-(2-isopentenyl) adenine (2iP) and 6-furfuryl aminopurine (Kn), differentiated multiple shoots from the axils. BA at 10μM proved optimum for elicitation of multiple shoots in 91.6% cultures with an average of 7.12 shoots per explant within 6weeks. The excised shoots rooted on half strength Murashige and Skoog's medium supplemented with 0.1μM IBA. Micropropagated plants were hardened and transferred to field for acclimatization, where 95% plants survived and were phenotypically similar to the donor plant. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were employed to evaluate the genetic fidelity amongst the regenerants. Eleven individuals, randomly chosen amongst a population of 120 regenerants were compared with the donor plant. A total of 71 scorable bands, ranging in size from 100bp to 1,100bp were generated by a combination of the two markers in the aforesaid plants. All banding profiles from micropropagated plants were monomorphic and similar to those of mother plant. The similarity values amongst the aforesaid plants varied from 0.967 to 1.000. The dendrogram generated through UPGMA (Unweighted Pair Group Method with arithmetic mean) analysis revealed 98% similarity amongst them, thus confirming the genetic fidelity of the in vitro clones. Prof. H.S. Srivastava Foundation for Science and Society, 2012 Cytokinin nationallicence Fidelity nationallicence ISSR nationallicence Micropropagation nationallicence RAPD nationallicence Spilanthes calva DC nationallicence 2iP : N6-(2-isopentenyl) adenine nationallicence BA : N6-benzyladenine nationallicence CTAB : Cetyl trimethyl ammonium bromide nationallicence DMRT : Duncan's multiple range test nationallicence IAA : Indole-3-acetic acid nationallicence IBA : Indole-3-butyric acid nationallicence ISSR : Inter simple sequence repeat nationallicence Kn : 6-furfuryl aminopurine nationallicence MS : Murashige and Skoog nationallicence NAA : α-naphthalene acetic acid nationallicence NTSYS : Numerical taxonomy and multivariate analysis system nationallicence RAPD : Random amplified polymorphic DNA nationallicence SAHN : Sequential agglomerative hierarchical and nested clustering method program in NTSYS nationallicence SIMQUAL : Similarity for qualitative data program in NTSYS nationallicence SPSS : Statistical package for social sciences nationallicence UPGMA : Unweighted pair group method with arithmetic mean nationallicence Razaq Mohd Department of Botany, University of Delhi, 110007, Delhi, India aut Heikrujam Monika Department of Botany, University of Delhi, 110007, Delhi, India aut Chetri Siva Department of Botany, University of Delhi, 110007, Delhi, India aut Agrawal Veena Department of Botany, University of Delhi, 110007, Delhi, India aut Physiology and Molecular Biology of Plants Springer-Verlag 19/2(2013-04-01), 251-260 0971-5894 19:2<251 2013 19 12298 https://doi.org/10.1007/s12298-012-0152-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-012-0152-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Razaq Mohd Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Heikrujam Monika Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Chetri Siva Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Agrawal Veena Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer-Verlag 19/2(2013-04-01), 251-260 0971-5894 19:2<251 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer