naa a22 4500 510788831 CHVBK 20180411083312.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s12298-012-0161-3 doi (NATIONALLICENCE)springer-10.1007/s12298-012-0161-3 Micropropagation of Ajuga bracteosa , a medicinal herb [Elektronische Daten] [Shivanee Kaul, Sandip Das, P. Srivastava] For conservation and genetic transformation, a successful in vitro micropropagation protocol for Ajuga bracteosa, a medicinal herb has been established for the first time. MS medium supplemented with IAA (2mg/L) and BA (5mg/L) induced 100% shoot regeneration with an average of 41.4 shoots of 8.4cm per culture. Excised in vitro shoots when transferred to MS + IBA (0.5mg/L) produced 20 roots/shoot of 20.2cm average length in 100% cultures. Of the three explants, leaf, petiole and root, leaf displayed quickest response followed by petiole while root was the slowest. Hardening of plantlets was achieved with 82% survival. The hardened plants were maintained in pots with garden soil under controlled (Temp. 25 ± 2°C) conditions. RAPD exhibited genetic fidelity with 100% monomorphism in regenerants. Prof. H.S. Srivastava Foundation for Science and Society, 2013 Ajuga bracteosa nationallicence Micropropagation nationallicence Medicinal plant nationallicence RAPD nationallicence Kaul Shivanee Department of Biotechnology, Jamia Hamdard, 110062, New Delhi, India aut Das Sandip Department of Botany, University of Delhi, 110 007, New Delhi, India aut Srivastava P. Department of Biotechnology, Faculty of Science, Hamdard University, 110062, New Delhi, India aut Physiology and Molecular Biology of Plants Springer-Verlag 19/2(2013-04-01), 289-296 0971-5894 19:2<289 2013 19 12298 https://doi.org/10.1007/s12298-012-0161-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-012-0161-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kaul Shivanee Department of Biotechnology, Jamia Hamdard, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Das Sandip Department of Botany, University of Delhi, 110 007, New Delhi, India aut NATIONALLICENCE 700 1- Srivastava P. Department of Biotechnology, Faculty of Science, Hamdard University, 110062, New Delhi, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer-Verlag 19/2(2013-04-01), 289-296 0971-5894 19:2<289 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer