naa a22 4500 51078884X CHVBK 20180411083312.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s12298-013-0162-x doi (NATIONALLICENCE)springer-10.1007/s12298-013-0162-x In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane ( Saccharum officinarum L.) [Elektronische Daten] [Srinath Rao, Jabeen FTZ] A system for in vitro selection of drought tolerant callus lines in sugarcane was developed. High molecular weight PEG was used as selective agent. Selected callus line grew better than non-selected callus when grown on different concentrations of PEG. The activity of antioxidant enzymes like CAT, POX, APX and SOD were high in selected callus than in non-selected callus. Osmolytes like proline and ascorbic acid were at higher levels in selected callus than in non-selected callus, however at higher concentrations (20-30%) of PEG, levels of proline and ascorbic acid decreased. The frequency of organogenesis and number of plantlets decreased in selected callus than in non-selected callus. The results can be used for in vitro screening and manipulations of sugarcane for improvement of drought tolerance Prof. H.S. Srivastava Foundation for Science and Society, 2013 Antioxidant enzymes nationallicence Callus nationallicence PEG nationallicence Selection nationallicence Proline nationallicence Sugarcane nationallicence 2,4-D : 2,4-dichloro phenoxy acitic acid nationallicence BAP : 6-Benzyl aminopurin nationallicence NAA : α-Napthalene acitic acid nationallicence Kn : Kinetin nationallicence CAT : Catalase nationallicence POX : Peroxidase nationallicence APX : Ascorbate peroxidase nationallicence SOD : Superoxide dismutase nationallicence MS : Murashige and Skoog nationallicence ROS : Reactive Oxygen Species nationallicence Rao Srinath Department of Botany, Gulbarga University, Gulbarga, India aut FTZ Jabeen Department of Botany, Gulbarga University, Gulbarga, India aut Physiology and Molecular Biology of Plants Springer-Verlag 19/2(2013-04-01), 261-268 0971-5894 19:2<261 2013 19 12298 https://doi.org/10.1007/s12298-013-0162-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12298-013-0162-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rao Srinath Department of Botany, Gulbarga University, Gulbarga, India aut NATIONALLICENCE 700 1- FTZ Jabeen Department of Botany, Gulbarga University, Gulbarga, India aut NATIONALLICENCE 773 0- Physiology and Molecular Biology of Plants Springer-Verlag 19/2(2013-04-01), 261-268 0971-5894 19:2<261 2013 19 12298 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer