naa a22 4500 510799396 CHVBK 20180411083348.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s12028-011-9633-5 doi (NATIONALLICENCE)springer-10.1007/s12028-011-9633-5 The Prognostic Value of Brain Extracellular Fluid Nitric Oxide Metabolites After Traumatic Brain Injury [Elektronische Daten] [Martin Tisdall, Konrad Rejdak, Neil Kitchen, Martin Smith, Axel Petzold] Background: Nitric oxide (NO) is a compound with both protective and damaging effects on neurons. Quantification of NO metabolites in humans is limited by sample contamination with blood. In vivo cerebral microdialysis may offer an alternative approach as sampling of extracellular fluid (ECF) adjacent to neurons becomes possible. We investigate the prognostic value of brain ECF NO metabolites in patients with traumatic brain injury (TBI). Methods: A prospective case cohort of 195 ECF samples collected from 11 cases over 4days following TBI was collected. Nitrate and nitrite concentrations ([NO x ]) were quantified using a vanadium-based colorimetric assay. Results: Early ECF [NO x ] (<48h post TBI) were significantly higher in non-survivors (median 59.2μmol/l, n=7) compared to survivors (23.3μmol/l, n=4) (P=0.04). Late (48-96h) ECF [NO x ] remained higher in non-survivors (47.9μmol/l) compared to survivors (23.0μmol/l) but this was not significant (P=0.29). Receiver operator characteristic analysis shows an optimized cutoff level for ECF [NO x ] of 26.5μmol/l measured <48h post TBI for predicting non-survival (sensitivity 100%, specificity 75%). Conclusion: Early ECF NO x concentrations are of prognostic value after TBI. ECF NO x may be a useful biomarker for treatment trials targeted at nitric oxide metabolism. Springer Science+Business Media, LLC, 2011 Traumatic brain injury nationallicence Microdialysis nationallicence Nitric oxide nationallicence Neurocritical care nationallicence Neuromonitoring nationallicence Tisdall Martin Departments of Neuroanaesthesia and Neurocritical Care, The National Hospital for Neurology and Neurosurgery, Queen Square, WC1N 3BG, London, UK aut Rejdak Konrad Department of Neurology, Medical University of Lublin, Lublin, Poland aut Kitchen Neil Victor Horsley Department of Neurosurgery, The National Hospital for Neurology and Neurosurgery, Queen Square, WC1N 3BG, London, UK aut Smith Martin Departments of Neuroanaesthesia and Neurocritical Care, The National Hospital for Neurology and Neurosurgery, Queen Square, WC1N 3BG, London, UK aut Petzold Axel Departments of Neuroanaesthesia and Neurocritical Care, The National Hospital for Neurology and Neurosurgery, Queen Square, WC1N 3BG, London, UK aut Neurocritical Care Springer US; http://www.springer-ny.com 19/1(2013-08-01), 65-68 1541-6933 19:1<65 2013 19 12028 https://doi.org/10.1007/s12028-011-9633-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12028-011-9633-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tisdall Martin Departments of Neuroanaesthesia and Neurocritical Care, The National Hospital for Neurology and Neurosurgery, Queen Square, WC1N 3BG, London, UK aut NATIONALLICENCE 700 1- Rejdak Konrad Department of Neurology, Medical University of Lublin, Lublin, Poland aut NATIONALLICENCE 700 1- Kitchen Neil Victor Horsley Department of Neurosurgery, The National Hospital for Neurology and Neurosurgery, Queen Square, WC1N 3BG, London, UK aut NATIONALLICENCE 700 1- Smith Martin Departments of Neuroanaesthesia and Neurocritical Care, The National Hospital for Neurology and Neurosurgery, Queen Square, WC1N 3BG, London, UK aut NATIONALLICENCE 700 1- Petzold Axel Departments of Neuroanaesthesia and Neurocritical Care, The National Hospital for Neurology and Neurosurgery, Queen Square, WC1N 3BG, London, UK aut NATIONALLICENCE 773 0- Neurocritical Care Springer US; http://www.springer-ny.com 19/1(2013-08-01), 65-68 1541-6933 19:1<65 2013 19 12028 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer