naa a22 4500 51079985X CHVBK 20180411083350.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s12028-012-9768-z doi (NATIONALLICENCE)springer-10.1007/s12028-012-9768-z Infarct Volume After Hyperacute Infusion of Hypertonic Saline in a Rat Model of Acute Embolic Stroke [Elektronische Daten] [Alexander Papangelou, Thomas Toung, Allan Gottschalk, Marek Mirski, Raymond Koehler] Introduction: Hypertonic saline (HS) can treat cerebral edema arising from a number of pathologic conditions. However, physicians are reluctant to use it during the first 24h after stroke because of experimental evidence that it increases infarct volume when administered early after reperfusion. Here, we determined the effect of HS on infarct size in an embolic clot model without planned reperfusion. Methods: A clot was injected into the internal carotid artery of male Wistar rats to reduce perfusion in the middle cerebral artery territory to less than 40% of baseline, as monitored by laser-Doppler flowmetry. After 25min, rats were randomized to receive 10mL/kg of 7.5% HS (50:50 chloride:acetate) or normal saline (NS) followed by a 0.5mL/h infusion of the same solution for 22h. Results: Infarct volume was similar between NS and HS groups (in mm3: cortex 102±65mm3 vs. 93±49mm3, p=0.72; caudoputamenal complex 15±9mm3 vs. 21±14, p=0.22; total hemisphere 119±76mm3 vs. 114±62, p=0.88, respectively). Percent water content was unchanged in the infarcted hemisphere (NS 81.6±1.5%; HS 80.7±1.3%, p=0.16), whereas the HS-treated contralateral hemisphere was significantly dehydrated (NS 79.4±0.8%; HS 77.5±0.8%, p<0.01). Conclusions: HS reduced contralateral hemispheric water content but did not affect ipsilateral brain water content when compared to NS. Infarct volume was unaffected by HS administration at all evaluated locations. Springer Science+Business Media, LLC, 2012 Embolic stroke nationallicence Hypernatremia nationallicence Hypertonic saline nationallicence Normal saline nationallicence Tissue water content nationallicence Wistar rats nationallicence Papangelou Alexander Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, 600 North Wolfe Street, Meyer 8-140, 21287-7840, Baltimore, MD, USA aut Toung Thomas Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, 600 North Wolfe Street, Meyer 8-140, 21287-7840, Baltimore, MD, USA aut Gottschalk Allan Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, 600 North Wolfe Street, Meyer 8-140, 21287-7840, Baltimore, MD, USA aut Mirski Marek Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, 600 North Wolfe Street, Meyer 8-140, 21287-7840, Baltimore, MD, USA aut Koehler Raymond Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, 600 North Wolfe Street, Blalock 1404, 21287-4961, Baltimore, MD, USA aut Neurocritical Care Humana Press Inc 18/1(2013-02-01), 106-114 1541-6933 18:1<106 2013 18 12028 https://doi.org/10.1007/s12028-012-9768-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12028-012-9768-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Papangelou Alexander Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, 600 North Wolfe Street, Meyer 8-140, 21287-7840, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Toung Thomas Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, 600 North Wolfe Street, Meyer 8-140, 21287-7840, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Gottschalk Allan Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, 600 North Wolfe Street, Meyer 8-140, 21287-7840, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Mirski Marek Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, 600 North Wolfe Street, Meyer 8-140, 21287-7840, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Koehler Raymond Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, 600 North Wolfe Street, Blalock 1404, 21287-4961, Baltimore, MD, USA aut NATIONALLICENCE 773 0- Neurocritical Care Humana Press Inc 18/1(2013-02-01), 106-114 1541-6933 18:1<106 2013 18 12028 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer