naa a22 4500 510805477 CHVBK 20180411083416.0 cr unu---uuuuu 180411e20130101xx s 000 0 eng 10.1007/s12576-012-0227-6 doi (NATIONALLICENCE)springer-10.1007/s12576-012-0227-6 Expression of specific IGFBPs is associated with those of the proliferating and differentiating markers in regenerating rat plantaris muscle [Elektronische Daten] [Akihiko Yamaguchi, Kunihiro Sakuma, Takahiko Fujikawa, Isao Morita] To examine the relationship between specific insulin-like growth factor (IGF)-binding proteins (IGFBPs) and myogenesis during muscle regeneration in vivo, we measured mRNA expression of IGFBPs and myogenic markers in rat plantaris muscle after bupivacaine administration. IGF-I Ea, MGF, IGFBPs and myogenic marker mRNAs were analyzed 12, 24, 48 and 72h after bupivacaine injection. IGFBP-1, -2, -3 and -4 proteins were immunostained after the treatment. MGF, IGF-I Ea and IGFBP-4 mRNAs started to increase 12 or 24h after bupivacaine injection and increased further after that. IGFBP-1, -2, -3 and -4 proteins were strongly stained in the immature muscle fiber nuclei and the extracellular matrix after bupivacaine injection. PCNA, MyoD, IGFBP-2 and IGFBP-3 mRNAs increased at 12 or 24h and did not show further increases after that. Myogenin, p21, IGFBP-1 and IGFBP-5 mRNAs sharply increased after 72h. These results suggest that specific IGFBPs are individually expressed and differently associated with the expression of myogenic markers in regenerating muscles. The Physiological Society of Japan and Springer, 2012 Insulin-like growth factor-1 nationallicence Mechano growth factor nationallicence Insulin-like growth factor-binding proteins nationallicence Myogenic regulatory factors nationallicence Muscle regeneration nationallicence Yamaguchi Akihiko Department of Integrated Human Sciences, School of Dentistry, Health Sciences University of Hokkaido, 061-0293, Kanazawa, Ishikari-Tobetsu, Hokkaido, Japan aut Sakuma Kunihiro Research Center of Physical Fitness, Sports and Health, Toyohashi University of Technology, Toyohashi, Aichi, Japan aut Fujikawa Takahiko Department of Pharmaceutical Sciences, Suzuka University of Medical Science, Suzuka, Mie, Japan aut Morita Isao Department of Integrated Human Sciences, School of Nursing and Social Services, Health Sciences University of Hokkaido, Kanazawa, Ishikari-Tobetsu, Hokkaido, Japan aut The Journal of Physiological Sciences Springer Japan 63/1(2013-01-01), 71-77 1880-6546 63:1<71 2013 63 12576 https://doi.org/10.1007/s12576-012-0227-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-012-0227-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamaguchi Akihiko Department of Integrated Human Sciences, School of Dentistry, Health Sciences University of Hokkaido, 061-0293, Kanazawa, Ishikari-Tobetsu, Hokkaido, Japan aut NATIONALLICENCE 700 1- Sakuma Kunihiro Research Center of Physical Fitness, Sports and Health, Toyohashi University of Technology, Toyohashi, Aichi, Japan aut NATIONALLICENCE 700 1- Fujikawa Takahiko Department of Pharmaceutical Sciences, Suzuka University of Medical Science, Suzuka, Mie, Japan aut NATIONALLICENCE 700 1- Morita Isao Department of Integrated Human Sciences, School of Nursing and Social Services, Health Sciences University of Hokkaido, Kanazawa, Ishikari-Tobetsu, Hokkaido, Japan aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/1(2013-01-01), 71-77 1880-6546 63:1<71 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer