naa a22 4500 510805531 CHVBK 20180411083416.0 cr unu---uuuuu 180411e20130101xx s 000 0 eng 10.1007/s12576-012-0231-x doi (NATIONALLICENCE)springer-10.1007/s12576-012-0231-x An inactivated Pseudomonas aeruginosa medicament inhibits airway allergic inflammation and improves epithelial functions [Elektronische Daten] [Yurong Tan, Huijun Liu, Huihui Yang, Lili wang, Xiaoqun Qin] The features of asthma are airway hyperresponsiveness (AHR), excess production of Th2 cytokines, and eosinophil accumulation in the lungs. To investigate the antiasthmatic potential of an inactivated Pseudomonas aeruginosa medicament (PPA), as well as the underlying mechanism involved, we studied the effects of PPA on airway and epithelial functions. Airway resistance, cell enumeration, and IL-4, IFN-γ, and IL-17 secretion in bronchoalveolar lavage fluid were assayed on an OVA-sensitized AHR animal model. Flow cytometry was used to observe the effects of PPA on cell proliferation, real-time PCR was used to test the expressions of toll like receptor 4 and 5, and Th17 signal molecules Act1, NF-kB negative regulator A20, and western blot were used to detect NF-kB expression on cultured human bronchial epithelial cells (BECs). PPA-treated animals had suppressed airway resistance, eosinophil and lymphocytes infiltration, and IL-4 and IL-17 secretion. PPA can stimulate toll-like receptor-4 and 5 expressions, promote cell proliferation in normal and OVA-treated BECs, significantly decrease Act1 and NF-kB, and increase A20 expression in BECs treated by OVA. Our data suggest the therapeutic mechanism by which PPA effectively treats allergic inflammation on reductions of airway responsiveness, eosinophil infiltration, IL-4 and IL-17 secretion, and improvements of epithelial functions. The Physiological Society of Japan and Springer, 2012 Pseudomonas aeruginosa nationallicence Toll-like receptors nationallicence Airway hyperresponsiveness nationallicence Th2 cytokines nationallicence Tan Yurong Department of Basic Medicine, Xiangya School of Medicine, Central South University, 410078, Changsha, Hunan, China aut Liu Huijun Department of Basic Medicine, Xiangya School of Medicine, Central South University, 410078, Changsha, Hunan, China aut Yang Huihui Department of Basic Medicine, Xiangya School of Medicine, Central South University, 410078, Changsha, Hunan, China aut wang Lili Department of Basic Medicine, Xiangya School of Medicine, Central South University, 410078, Changsha, Hunan, China aut Qin Xiaoqun Department of Basic Medicine, Xiangya School of Medicine, Central South University, 410078, Changsha, Hunan, China aut The Journal of Physiological Sciences Springer Japan 63/1(2013-01-01), 63-69 1880-6546 63:1<63 2013 63 12576 https://doi.org/10.1007/s12576-012-0231-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-012-0231-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tan Yurong Department of Basic Medicine, Xiangya School of Medicine, Central South University, 410078, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Liu Huijun Department of Basic Medicine, Xiangya School of Medicine, Central South University, 410078, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Yang Huihui Department of Basic Medicine, Xiangya School of Medicine, Central South University, 410078, Changsha, Hunan, China aut NATIONALLICENCE 700 1- wang Lili Department of Basic Medicine, Xiangya School of Medicine, Central South University, 410078, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Qin Xiaoqun Department of Basic Medicine, Xiangya School of Medicine, Central South University, 410078, Changsha, Hunan, China aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/1(2013-01-01), 63-69 1880-6546 63:1<63 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer