naa a22 4500 51080554X CHVBK 20180411083416.0 cr unu---uuuuu 180411e20130101xx s 000 0 eng 10.1007/s12576-012-0230-y doi (NATIONALLICENCE)springer-10.1007/s12576-012-0230-y Angiotensin II induced differentially expressed microRNAs in adult rat cardiac fibroblasts [Elektronische Daten] [Xiaoying Jiang, Qilan Ning, Juanli Wang] Angiotensin II (Ang II) plays a pivotal role in cardiac fibrosis, and microRNAs (miRNAs) have been shown to participate in diverse pathological processes. Our aim is to identify the Ang II-induced miRNAs in cardiac fibroblasts (CFs). The miRNA array was used to analyze the miRNA expression profile in CFs treated by Ang II and control cells. Stem-loop real-time PCR was performed to re-measure the levels of the differentially expressed miRNAs. Analysis of miRNA arrays showed that 33 miRNAs were differentially expressed (13 up- and 20 downregulated) in response to Ang II (100nM) for 24h as compared to control cells. Quantitative PCR revealed that Ang II upregulated the levels of miR-132, -125b-3p and miR-146b but downregulated the levels of miR-300-5p, -204* and miR-181b in CFs. The trend of miRNA change is consistent with microarray and qRT-PCR. Bioinformatic analysis revealed that MMP9 as the target of miR-132, MMP16 as the target of miR-146b and TIMP3 as the target of miR-181b have been listed in the miR database with experimentally validated targets, indicating the potential role of those miRNAs in cardiac fibrosis. Our results demonstrated that we did identify a subset of miRNAs that was differentially expressed in Ang II-treated CFs, which provide a starting point to explore their potential roles in cardiac fibrosis and hypertension. The Physiological Society of Japan and Springer, 2012 Angiotensin II nationallicence Cardiac fibroblasts nationallicence miRNA nationallicence Cardiac fibrosis nationallicence Jiang Xiaoying Department of Genetics and Molecular Biology, School of Medicine, Xi'an Jiaotong University, 76 Yanta West Road, 710061, Xi'an, Shaanxi, China aut Ning Qilan Department of Genetics and Molecular Biology, School of Medicine, Xi'an Jiaotong University, 76 Yanta West Road, 710061, Xi'an, Shaanxi, China aut Wang Juanli Department of Genetics and Molecular Biology, School of Medicine, Xi'an Jiaotong University, 76 Yanta West Road, 710061, Xi'an, Shaanxi, China aut The Journal of Physiological Sciences Springer Japan 63/1(2013-01-01), 31-38 1880-6546 63:1<31 2013 63 12576 https://doi.org/10.1007/s12576-012-0230-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-012-0230-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jiang Xiaoying Department of Genetics and Molecular Biology, School of Medicine, Xi'an Jiaotong University, 76 Yanta West Road, 710061, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Ning Qilan Department of Genetics and Molecular Biology, School of Medicine, Xi'an Jiaotong University, 76 Yanta West Road, 710061, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Wang Juanli Department of Genetics and Molecular Biology, School of Medicine, Xi'an Jiaotong University, 76 Yanta West Road, 710061, Xi'an, Shaanxi, China aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/1(2013-01-01), 31-38 1880-6546 63:1<31 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer