naa a22 4500 510805590 CHVBK 20180411083416.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s12576-013-0277-4 doi (NATIONALLICENCE)springer-10.1007/s12576-013-0277-4 Immobilization-induced hypersensitivity associated with spinal cord sensitization during cast immobilization and after cast removal in rats [Elektronische Daten] [Yohei Hamaue, Jiro Nakano, Yuki Sekino, Sayaka Chuganji, Jyunya Sakamoto, Toshiro Yoshimura, Tomoki Origuchi, Minoru Okita] This study examined mechanical and thermal hypersensitivity in the rat hind paw during cast immobilization of the hind limbs for 4 or 8weeks and following cast removal. Blood flow, skin temperature, and volume of the rat hind paw were assessed in order to determine peripheral circulation of the hind limbs. Sensitization was analyzed by measuring the expression of the calcitonin gene-related peptide (CGRP) in the spinal dorsal horn following cast immobilization. Twoweeks post immobilization, mechanical and thermal sensitivities increased significantly in all rats; however, peripheral circulation was not affected by immobilization. Cast immobilization for 8weeks induced more serious hypersensitivity compared to cast immobilization for 4weeks. Moreover, CGRP expression in the deeper lamina layer of the spinal dorsal horn increased in the rats immobilized for 8weeks but not in those immobilized for 4weeks. These findings suggest that immobilization-induced hypersensitivity develops during the immobilization period without affecting peripheral circulation. Our results also highlight the possibility that prolonged immobilization induces central sensitization in the spinal cord. The Physiological Society of Japan and Springer Japan, 2013 Immobilization nationallicence Hypersensitivity nationallicence CGRP nationallicence Spinal cord nationallicence Rat nationallicence Hamaue Yohei Department of Locomotive Rehabilitation Science, Unit of Rehabilitation Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8520, Nagasaki, Japan aut Nakano Jiro Unit of Physical and Occupational Therapy Sciences, Nagasaki University Graduate School of Biochemical Sciences, 1-7-1 Sakamoto, 852-8520, Nagasaki, Japan aut Sekino Yuki Department of Locomotive Rehabilitation Science, Unit of Rehabilitation Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8520, Nagasaki, Japan aut Chuganji Sayaka Unit of Physical and Occupational Therapy Sciences, Nagasaki University Graduate School of Biochemical Sciences, 1-7-1 Sakamoto, 852-8520, Nagasaki, Japan aut Sakamoto Jyunya Department of Rehabilitation, Nagasaki University Hospital, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan aut Yoshimura Toshiro Department of Locomotive Rehabilitation Science, Unit of Rehabilitation Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8520, Nagasaki, Japan aut Origuchi Tomoki Department of Locomotive Rehabilitation Science, Unit of Rehabilitation Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8520, Nagasaki, Japan aut Okita Minoru Department of Locomotive Rehabilitation Science, Unit of Rehabilitation Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8520, Nagasaki, Japan aut The Journal of Physiological Sciences Springer Japan 63/6(2013-11-01), 401-408 1880-6546 63:6<401 2013 63 12576 https://doi.org/10.1007/s12576-013-0277-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-013-0277-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hamaue Yohei Department of Locomotive Rehabilitation Science, Unit of Rehabilitation Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8520, Nagasaki, Japan aut NATIONALLICENCE 700 1- Nakano Jiro Unit of Physical and Occupational Therapy Sciences, Nagasaki University Graduate School of Biochemical Sciences, 1-7-1 Sakamoto, 852-8520, Nagasaki, Japan aut NATIONALLICENCE 700 1- Sekino Yuki Department of Locomotive Rehabilitation Science, Unit of Rehabilitation Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8520, Nagasaki, Japan aut NATIONALLICENCE 700 1- Chuganji Sayaka Unit of Physical and Occupational Therapy Sciences, Nagasaki University Graduate School of Biochemical Sciences, 1-7-1 Sakamoto, 852-8520, Nagasaki, Japan aut NATIONALLICENCE 700 1- Sakamoto Jyunya Department of Rehabilitation, Nagasaki University Hospital, 1-7-1 Sakamoto, 852-8501, Nagasaki, Japan aut NATIONALLICENCE 700 1- Yoshimura Toshiro Department of Locomotive Rehabilitation Science, Unit of Rehabilitation Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8520, Nagasaki, Japan aut NATIONALLICENCE 700 1- Origuchi Tomoki Department of Locomotive Rehabilitation Science, Unit of Rehabilitation Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8520, Nagasaki, Japan aut NATIONALLICENCE 700 1- Okita Minoru Department of Locomotive Rehabilitation Science, Unit of Rehabilitation Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, 852-8520, Nagasaki, Japan aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/6(2013-11-01), 401-408 1880-6546 63:6<401 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer