naa a22 4500 510805639 CHVBK 20180411083417.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s12576-013-0283-6 doi (NATIONALLICENCE)springer-10.1007/s12576-013-0283-6 High-fat diet induced an oxidative stress in white adipose tissue and disturbed plasma transition metals in rat: prevention by grape seed and skin extract [Elektronische Daten] [Kamel Charradi, Salem Elkahoui, Ferid Limam, Ezzedine Aouani] Obesity is a public health problem characterized by increased accumulation of fat into adipose tissues leading to oxidative stress, dyslipidemia, and chronic inflammatory status. We used an experimental model of high-fat diet-induced obesity to analyze the link between dyslipidemia, oxidative stress, and fat accumulation into adipose tissue of rats, as well as the involvement of intracellular mediators such as transition metals on signal transduction. We also looked at the ability of a grape seed and skin extract (GSSE) from a Tunisian cultivar to prevent fat-induced disturbances. Data showed that a high-fat diet (HFD) provoked dyslipidemia into plasma which is linked to an oxidative stress, an accumulation of transition metals such as manganese, copper, and zinc and a depletion of iron. GSSE prevented dyslipidemia by modulating lipase activity, together with increased antioxidant capacity and depletion of transition metals as well as of free radicals such as O2 − and OH. These data indicated that GSSE has important preventive effects on HFD-induced obesity and oxidative stress whose transduction seems to involve transition metals. GSSE should be used as a safe anti-obesity agent that could find potential applications in metabolic disorders involving transition metals dyshomeostasis. The Physiological Society of Japan and Springer Japan, 2013 Dyslipidemia nationallicence Grape polyphenols nationallicence Oxidative stress nationallicence Plasma nationallicence White adipose tissue nationallicence EAT : Epididymal adipose tissue nationallicence GSSE : Grape seed and skin extract nationallicence HFD : High-fat diet nationallicence MAT : Mesenteric adipose tissue nationallicence PAT : Perirenal adipose tissue nationallicence RAT : Retroperitoneal adipose tissue nationallicence Charradi Kamel Laboratoire des Substances Bioactives (LSBA), Centre de Biotechnologie de Borj Cedria, BP-901, 2050, Hammam-Lif, Tunisia aut Elkahoui Salem Laboratoire des Substances Bioactives (LSBA), Centre de Biotechnologie de Borj Cedria, BP-901, 2050, Hammam-Lif, Tunisia aut Limam Ferid Laboratoire des Substances Bioactives (LSBA), Centre de Biotechnologie de Borj Cedria, BP-901, 2050, Hammam-Lif, Tunisia aut Aouani Ezzedine Laboratoire des Substances Bioactives (LSBA), Centre de Biotechnologie de Borj Cedria, BP-901, 2050, Hammam-Lif, Tunisia aut The Journal of Physiological Sciences Springer Japan 63/6(2013-11-01), 445-455 1880-6546 63:6<445 2013 63 12576 https://doi.org/10.1007/s12576-013-0283-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-013-0283-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Charradi Kamel Laboratoire des Substances Bioactives (LSBA), Centre de Biotechnologie de Borj Cedria, BP-901, 2050, Hammam-Lif, Tunisia aut NATIONALLICENCE 700 1- Elkahoui Salem Laboratoire des Substances Bioactives (LSBA), Centre de Biotechnologie de Borj Cedria, BP-901, 2050, Hammam-Lif, Tunisia aut NATIONALLICENCE 700 1- Limam Ferid Laboratoire des Substances Bioactives (LSBA), Centre de Biotechnologie de Borj Cedria, BP-901, 2050, Hammam-Lif, Tunisia aut NATIONALLICENCE 700 1- Aouani Ezzedine Laboratoire des Substances Bioactives (LSBA), Centre de Biotechnologie de Borj Cedria, BP-901, 2050, Hammam-Lif, Tunisia aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/6(2013-11-01), 445-455 1880-6546 63:6<445 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer