naa a22 4500 510805647 CHVBK 20180411083417.0 cr unu---uuuuu 180411e20131101xx s 000 0 eng 10.1007/s12576-013-0281-8 doi (NATIONALLICENCE)springer-10.1007/s12576-013-0281-8 ABCG1 deficiency promotes endothelial apoptosis by endoplasmic reticulum stress-dependent pathway [Elektronische Daten] [Jiahong Xue, Jin Wei, Xin Dong, Canzhan Zhu, Yongqin Li, Anqi Song, Zhongwei Liu] The present study was focused on whether ABCG1 deficiency was involved in endothelial apoptosis and its possible mechanism. Human umbilical artery endothelial cells were transfected with ABCG1 siRNA and/or ABCG1 expression plasmid. We observed that silencing of endothelial ABCG1 reduced cholesterol efflux to HDL and increased intracellular lipid content. Moreover, reduction of ABCG1 promoted endothelial apoptosis and expression of endoplasmic reticulum (ER) stress-related molecules GRP78 and CHOP. In contrast, transfection of ABCG1 overexpression plasmid reversed endothelial apoptosis and intracellular lipid accumulation as well as decreased expression of GRP78 and CHOP in ABCG1-deficient endothelial cells. Furthermore, endothelial apoptosis and ER stress-related molecules were induced by repletion of endothelial cells with cholesterol-loaded cyclodextrin, otherwise endothelial apoptotic response and expression of GRP78 and CHOP were suppressed by depletion of cellular cholesterol in ABCG1-deficient endothelial cells. The present results suggest that reduction of ABCG1 induces endothelial apoptosis, which seems associated with intracellular free cholesterol accumulation and subsequent ER stress. The Physiological Society of Japan and Springer Japan, 2013 ATP-binding cassette transporter G1 nationallicence Endothelial apoptosis nationallicence Endoplasmic reticulum stress nationallicence Cholesterol efflux nationallicence Xue Jiahong Department of Cardiovascular Medicine, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, 157 West Five Road, 710004, Xi'an, Shaanxi, China aut Wei Jin Department of Cardiovascular Medicine, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, 157 West Five Road, 710004, Xi'an, Shaanxi, China aut Dong Xin Department of Cardiovascular Medicine, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, 157 West Five Road, 710004, Xi'an, Shaanxi, China aut Zhu Canzhan Department of Cardiovascular Medicine, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, 157 West Five Road, 710004, Xi'an, Shaanxi, China aut Li Yongqin Department of Cardiovascular Medicine, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, 157 West Five Road, 710004, Xi'an, Shaanxi, China aut Song Anqi Department of Cardiovascular Medicine, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, 157 West Five Road, 710004, Xi'an, Shaanxi, China aut Liu Zhongwei Department of Cardiovascular Medicine, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, 157 West Five Road, 710004, Xi'an, Shaanxi, China aut The Journal of Physiological Sciences Springer Japan 63/6(2013-11-01), 435-444 1880-6546 63:6<435 2013 63 12576 https://doi.org/10.1007/s12576-013-0281-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-013-0281-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xue Jiahong Department of Cardiovascular Medicine, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, 157 West Five Road, 710004, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Wei Jin Department of Cardiovascular Medicine, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, 157 West Five Road, 710004, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Dong Xin Department of Cardiovascular Medicine, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, 157 West Five Road, 710004, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Zhu Canzhan Department of Cardiovascular Medicine, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, 157 West Five Road, 710004, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Li Yongqin Department of Cardiovascular Medicine, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, 157 West Five Road, 710004, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Song Anqi Department of Cardiovascular Medicine, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, 157 West Five Road, 710004, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Liu Zhongwei Department of Cardiovascular Medicine, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, 157 West Five Road, 710004, Xi'an, Shaanxi, China aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/6(2013-11-01), 435-444 1880-6546 63:6<435 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer