naa a22 4500 510805655 CHVBK 20180411083417.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s12576-013-0260-0 doi (NATIONALLICENCE)springer-10.1007/s12576-013-0260-0 Infection of primary hepatocytes with adenoviral vectors alters biliary lipid metabolism [Elektronische Daten] [Yuri Rueda, Itsaso Garcia-Arcos, Patricia Aspichueta, Begoña Ochoa, Lourdes Palacios, Olatz Fresnedo] In the context of a study of the involvement of SND1 (also known as coactivator p100) in biliary lipid secretion by primary rat hepatocytes, first-generation adenoviral vectors were used to promote the overexpression and underexpression of the protein SND1. Although differential expression of SND1 did not result in significant changes in the processes studied, some effects of the adenoviral infection itself were observed. In particular, infected hepatocytes showed a higher intracellular taurocholate accumulation capacity. Additionally, small heterodimer partner (SHP) and farnesoid X receptor (FXR), which are nuclear receptors essential for the regulation of bile salt metabolism and transport, were underregulated at the mRNA level. Our results suggest that adenoviral vectors could be altering some important control mechanism and indicate that adenoviral vectors should be used with caution as transfection vectors for hepatocytes when biliary lipid metabolism is to be studied. The Physiological Society of Japan and Springer Japan, 2013 Adenoviral vector nationallicence Bile salts nationallicence Primary hepatocytes nationallicence SHP nationallicence FXR nationallicence SND1 nationallicence Rueda Yuri Department of Physiology, Faculty of Medicine and Dentistry of the University of the Basque Country UPV/EHU, Bº Sarriena s/n 48940 Leioa, Bizkaia, Spain aut Garcia-Arcos Itsaso Department of Physiology, Faculty of Medicine and Dentistry of the University of the Basque Country UPV/EHU, Bº Sarriena s/n 48940 Leioa, Bizkaia, Spain aut Aspichueta Patricia Department of Physiology, Faculty of Medicine and Dentistry of the University of the Basque Country UPV/EHU, Bº Sarriena s/n 48940 Leioa, Bizkaia, Spain aut Ochoa Begoña Department of Physiology, Faculty of Medicine and Dentistry of the University of the Basque Country UPV/EHU, Bº Sarriena s/n 48940 Leioa, Bizkaia, Spain aut Palacios Lourdes Department of Physiology, Faculty of Medicine and Dentistry of the University of the Basque Country UPV/EHU, Bº Sarriena s/n 48940 Leioa, Bizkaia, Spain aut Fresnedo Olatz Department of Physiology, Faculty of Medicine and Dentistry of the University of the Basque Country UPV/EHU, Bº Sarriena s/n 48940 Leioa, Bizkaia, Spain aut The Journal of Physiological Sciences Springer Japan 63/3(2013-05-01), 225-229 1880-6546 63:3<225 2013 63 12576 https://doi.org/10.1007/s12576-013-0260-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-013-0260-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rueda Yuri Department of Physiology, Faculty of Medicine and Dentistry of the University of the Basque Country UPV/EHU, Bº Sarriena s/n 48940 Leioa, Bizkaia, Spain aut NATIONALLICENCE 700 1- Garcia-Arcos Itsaso Department of Physiology, Faculty of Medicine and Dentistry of the University of the Basque Country UPV/EHU, Bº Sarriena s/n 48940 Leioa, Bizkaia, Spain aut NATIONALLICENCE 700 1- Aspichueta Patricia Department of Physiology, Faculty of Medicine and Dentistry of the University of the Basque Country UPV/EHU, Bº Sarriena s/n 48940 Leioa, Bizkaia, Spain aut NATIONALLICENCE 700 1- Ochoa Begoña Department of Physiology, Faculty of Medicine and Dentistry of the University of the Basque Country UPV/EHU, Bº Sarriena s/n 48940 Leioa, Bizkaia, Spain aut NATIONALLICENCE 700 1- Palacios Lourdes Department of Physiology, Faculty of Medicine and Dentistry of the University of the Basque Country UPV/EHU, Bº Sarriena s/n 48940 Leioa, Bizkaia, Spain aut NATIONALLICENCE 700 1- Fresnedo Olatz Department of Physiology, Faculty of Medicine and Dentistry of the University of the Basque Country UPV/EHU, Bº Sarriena s/n 48940 Leioa, Bizkaia, Spain aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/3(2013-05-01), 225-229 1880-6546 63:3<225 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer