naa a22 4500 510805663 CHVBK 20180411083417.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s12576-013-0253-z doi (NATIONALLICENCE)springer-10.1007/s12576-013-0253-z Intracellular β2-adrenergic receptor signaling specificity in mouse skeletal muscle in response to single-dose β2-agonist clenbuterol treatment and acute exercise [Elektronische Daten] [Shogo Sato, Ken Shirato, Ryosuke Mitsuhashi, Daisuke Inoue, Takako Kizaki, Hideki Ohno, Kaoru Tachiyashiki, Kazuhiko Imaizumi] The aim of this study was to clarify the intracellular β2-adrenergic receptor signaling specificity in mouse slow-twitch soleus and fast-twitch tibialis anterior (TA) muscles, resulting from single-dose β2-agonist clenbuterol treatment and acute exercise. At 1, 4, and 24h after single-dose treatment with clenbuterol or after acute running exercise, the soleus and TA muscles were isolated and subjected to analysis. The phosphorylation of p38 mitogen-activated protein kinase (MAPK) increased after single-dose clenbuterol treatment and acute exercise in the soleus muscle but not in the TA muscle. Although there was no change in the phosphorylation of Akt after acute exercise in either muscle, phosphorylation of Akt in the soleus muscle increased after single-dose clenbuterol treatment, whereas that in the TA muscle remained unchanged. These results suggest that p38 MAPK and Akt pathways play a functional role in the adaptation to clenbuterol treatment and exercise, particularly in slow-twitch muscles. The Author(s), 2013 β2-Agonist clenbuterol nationallicence Exercise nationallicence Skeletal muscle nationallicence β2-Adrenergic receptor signaling nationallicence p38 MAPK nationallicence Akt nationallicence Sato Shogo Laboratory of Physiological Sciences, Faculty of Human Sciences, Waseda University, 2-579-15 Mikajima, 359-1192, Tokorozawa, Saitama, Japan aut Shirato Ken Laboratory of Physiological Sciences, Faculty of Human Sciences, Waseda University, 2-579-15 Mikajima, 359-1192, Tokorozawa, Saitama, Japan aut Mitsuhashi Ryosuke Laboratory of Physiological Sciences, Faculty of Human Sciences, Waseda University, 2-579-15 Mikajima, 359-1192, Tokorozawa, Saitama, Japan aut Inoue Daisuke Laboratory of Physiological Sciences, Faculty of Human Sciences, Waseda University, 2-579-15 Mikajima, 359-1192, Tokorozawa, Saitama, Japan aut Kizaki Takako Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, 6-20-2 Shinkawa, 181-8611, Mitaka, Tokyo, Japan aut Ohno Hideki Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, 6-20-2 Shinkawa, 181-8611, Mitaka, Tokyo, Japan aut Tachiyashiki Kaoru Department of Natural and Living Sciences, Graduate School of Education, Joetsu University of Education, 1 Yamayashiki, 943-8512, Joetsu, Niigata, Japan aut Imaizumi Kazuhiko Laboratory of Physiological Sciences, Faculty of Human Sciences, Waseda University, 2-579-15 Mikajima, 359-1192, Tokorozawa, Saitama, Japan aut The Journal of Physiological Sciences Springer Japan 63/3(2013-05-01), 211-218 1880-6546 63:3<211 2013 63 12576 https://doi.org/10.1007/s12576-013-0253-z text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-013-0253-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sato Shogo Laboratory of Physiological Sciences, Faculty of Human Sciences, Waseda University, 2-579-15 Mikajima, 359-1192, Tokorozawa, Saitama, Japan aut NATIONALLICENCE 700 1- Shirato Ken Laboratory of Physiological Sciences, Faculty of Human Sciences, Waseda University, 2-579-15 Mikajima, 359-1192, Tokorozawa, Saitama, Japan aut NATIONALLICENCE 700 1- Mitsuhashi Ryosuke Laboratory of Physiological Sciences, Faculty of Human Sciences, Waseda University, 2-579-15 Mikajima, 359-1192, Tokorozawa, Saitama, Japan aut NATIONALLICENCE 700 1- Inoue Daisuke Laboratory of Physiological Sciences, Faculty of Human Sciences, Waseda University, 2-579-15 Mikajima, 359-1192, Tokorozawa, Saitama, Japan aut NATIONALLICENCE 700 1- Kizaki Takako Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, 6-20-2 Shinkawa, 181-8611, Mitaka, Tokyo, Japan aut NATIONALLICENCE 700 1- Ohno Hideki Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, 6-20-2 Shinkawa, 181-8611, Mitaka, Tokyo, Japan aut NATIONALLICENCE 700 1- Tachiyashiki Kaoru Department of Natural and Living Sciences, Graduate School of Education, Joetsu University of Education, 1 Yamayashiki, 943-8512, Joetsu, Niigata, Japan aut NATIONALLICENCE 700 1- Imaizumi Kazuhiko Laboratory of Physiological Sciences, Faculty of Human Sciences, Waseda University, 2-579-15 Mikajima, 359-1192, Tokorozawa, Saitama, Japan aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/3(2013-05-01), 211-218 1880-6546 63:3<211 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer