naa a22 4500 510805728 CHVBK 20180411083417.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s12576-012-0244-5 doi (NATIONALLICENCE)springer-10.1007/s12576-012-0244-5 Stage-dependent anti-allodynic effects of intrathecal Toll-like receptor 4 antagonists in a rat model of cancer induced bone pain [Elektronische Daten] [Xiu Li, Xiao-Wei Wang, Xiao-Ming Feng, Wen-Jun Zhou, Yan-Qing Wang, Qi-Liang Mao-Ying] It has been reported that Toll-like receptor 4 (TLR4), which plays an important role in glial activation in neuropathic pain, is significantly increased in cancer pain. The present study was designed to assess the role of TLR4 in cancer-induced bone pain (CIBP) by intrathecal administration of TLR4 signaling pathway blocker naloxone or lipopolysaccharide Rhodobacter sphaeroides (LPS-RS). The rats developed significant mechanical allodynia from day 8 after intratibial Walker 256 inoculation. Intrathecal injection of naloxone or LPS-RS at day 8 significantly attenuated mechanical allodynia as shown by increased paw withdrawal thresholds. In contrast, the same pharmacological treatment showed no or slight pain relieving effect at day 16. Our findings demonstrate that the spinal TLR4 signaling pathway contributes to the mechanism underlying CIBP in a stage-dependent manner in rats, and it may be an efficacious target at early stage for the treatment in the future. The Physiological Society of Japan and Springer Japan, 2013 Cancer-induced bone pain nationallicence Toll-like receptor 4 nationallicence Spinal cord nationallicence Glia nationallicence Li Xiu Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Institute of Acupuncture Research, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, Fudan University, 138 Yi-Xue-Yuan Road, P.O. Box 291, 200032, Shanghai, People's Republic of China aut Wang Xiao-Wei Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Institute of Acupuncture Research, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, Fudan University, 138 Yi-Xue-Yuan Road, P.O. Box 291, 200032, Shanghai, People's Republic of China aut Feng Xiao-Ming Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Institute of Acupuncture Research, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, Fudan University, 138 Yi-Xue-Yuan Road, P.O. Box 291, 200032, Shanghai, People's Republic of China aut Zhou Wen-Jun Integrative Immunology and Behavior Program, College of ACES and College of Medicine, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA aut Wang Yan-Qing Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Institute of Acupuncture Research, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, Fudan University, 138 Yi-Xue-Yuan Road, P.O. Box 291, 200032, Shanghai, People's Republic of China aut Mao-Ying Qi-Liang Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Institute of Acupuncture Research, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, Fudan University, 138 Yi-Xue-Yuan Road, P.O. Box 291, 200032, Shanghai, People's Republic of China aut The Journal of Physiological Sciences Springer Japan 63/3(2013-05-01), 203-209 1880-6546 63:3<203 2013 63 12576 https://doi.org/10.1007/s12576-012-0244-5 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-012-0244-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Xiu Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Institute of Acupuncture Research, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, Fudan University, 138 Yi-Xue-Yuan Road, P.O. Box 291, 200032, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wang Xiao-Wei Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Institute of Acupuncture Research, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, Fudan University, 138 Yi-Xue-Yuan Road, P.O. Box 291, 200032, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Feng Xiao-Ming Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Institute of Acupuncture Research, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, Fudan University, 138 Yi-Xue-Yuan Road, P.O. Box 291, 200032, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Wen-Jun Integrative Immunology and Behavior Program, College of ACES and College of Medicine, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Wang Yan-Qing Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Institute of Acupuncture Research, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, Fudan University, 138 Yi-Xue-Yuan Road, P.O. Box 291, 200032, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Mao-Ying Qi-Liang Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Institute of Acupuncture Research, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, Fudan University, 138 Yi-Xue-Yuan Road, P.O. Box 291, 200032, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/3(2013-05-01), 203-209 1880-6546 63:3<203 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer