naa a22 4500 510805760 CHVBK 20180411083417.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12576-013-0268-5 doi (NATIONALLICENCE)springer-10.1007/s12576-013-0268-5 Radial stability of the actomyosin filament lattice in isolated skeletal myofibrils studied using atomic force microscopy [Elektronische Daten] [Daisuke Miyashiro, Jun'ichi Wakayama, Nao Akiyama, Yuki Kunioka, Takenori Yamada] The radial stability of the actomyosin filament lattice in skeletal myofibrils was examined by using atomic force microscopy. The diameter and the radial stiffness of the A-band region were examined based on force-distance curves obtained for single myofibrils adsorbed onto cover slips and compressed with the tip of a cantilever and with the Dextran treatment. The results obtained indicated that the A-band is composed of a couple of stiffness components having a rigid core-like component. It was further clarified that these radial components changed the thickness as well as the stiffness depending on the physiological condition of myofibrils. Notably, by decreasing the ionic strength, the diameter of the A-band region became greatly shrunken, but the rigid core-like component thickened, indicating that the electrostatic force distinctly affects the radial structure of actomyosin filament components. The results obtained were analyzed based on the elementary structures of the filament lattice composed of cross-bridges, thin filaments and thick filament backbones. It was clarified that the actomyosin filament lattice is radially deformable greatly and that (1), under mild compression, the filament lattice is stabilized primarily by the interactions of myosin heads with thin filaments and thick filament backbones, and (2), under severe compression, the electrostatic repulsive interactions between thin filaments and thick filament backbones became predominant. The Physiological Society of Japan and Springer Japan, 2013 Skeletal myofibril nationallicence Actomyosin filament nationallicence Lattice stability nationallicence Electrostatic force nationallicence Atomic force microscopy nationallicence AFM : Atomic force microscopy nationallicence AMPPNP : Adenyl-5′-yl-imidodiphosphate nationallicence EGTA : Ethylene glycol tetraacetic acid nationallicence PIPES : Piperazine-1,4-bis(2-ethanesulfonic acid) nationallicence Miyashiro Daisuke Department of Physics (Biophysics Section), Tokyo University of Science, Tokyo, Japan aut Wakayama Jun'ichi Nanobiotechnology Laboratory (Food Engineering Division), National Food Research Institute, National Agriculture and Food Research Organization, Ibaraki, Japan aut Akiyama Nao Department of Physics (Biophysics Section), Tokyo University of Science, Tokyo, Japan aut Kunioka Yuki Japan Science and Technology Agency, Innovation Plaza Ishikawa, Ishikawa, Japan aut Yamada Takenori Department of Physics (Biophysics Section), Tokyo University of Science, Tokyo, Japan aut The Journal of Physiological Sciences Springer Japan 63/4(2013-07-01), 299-310 1880-6546 63:4<299 2013 63 12576 https://doi.org/10.1007/s12576-013-0268-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-013-0268-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miyashiro Daisuke Department of Physics (Biophysics Section), Tokyo University of Science, Tokyo, Japan aut NATIONALLICENCE 700 1- Wakayama Jun'ichi Nanobiotechnology Laboratory (Food Engineering Division), National Food Research Institute, National Agriculture and Food Research Organization, Ibaraki, Japan aut NATIONALLICENCE 700 1- Akiyama Nao Department of Physics (Biophysics Section), Tokyo University of Science, Tokyo, Japan aut NATIONALLICENCE 700 1- Kunioka Yuki Japan Science and Technology Agency, Innovation Plaza Ishikawa, Ishikawa, Japan aut NATIONALLICENCE 700 1- Yamada Takenori Department of Physics (Biophysics Section), Tokyo University of Science, Tokyo, Japan aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/4(2013-07-01), 299-310 1880-6546 63:4<299 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer