naa a22 4500 510805795 CHVBK 20180411083417.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12576-013-0255-x doi (NATIONALLICENCE)springer-10.1007/s12576-013-0255-x Detection of dihydropyridine- and voltage-sensitive intracellular Ca2+ signals in normal human parathyroid cells [Elektronische Daten] [Rinako Iida, Keitaro Yokoyama, Ichiro Ohkido, Isao Tabei, Hiroshi Takeyama, Akifumi Suzuki, Toshiaki Shibasaki, Douchi Matsuba, Norio Suda, Tatsuo Hosoya] We recently showed dihydropyridine- and voltage-sensitive Ca2+ entry in cultured parathyroid cells from patients with secondary hyperparathyroidism. To determine whether normal parathyroid cells have a similar extracellular Ca2+ entry system, cells were isolated from normal (non-hyperplastic) human parathyroid glands. Fluorescence signals related to the cytoplasmic Ca2+ concentration ([Ca2+]I) were examined in these cells. Cells loaded with fluo-3/AM showed a transient increase in fluorescence (Ca2+ transient) following a 10-s exposure to a 150mM K+ solution in the presence of millimolar concentrations of external Ca2+. The Ca2+ transient was reduced by dihydropyridine antagonists or 0.5mM Cd2+, but enhanced by FPL-64176, an L-type Ca2+-channel agonist. Ca2+ transients induced by the 10-s exposure to 3.0mM extracellular Ca2+ ([Ca2+]o) were also inhibited by dihydropyridine antagonists or 0.5mM Cd2+. These results provide the first evidence that normal human parathyroid cells express a dihydropyridine-sensitive Ca2+ entry system that may be involved in the [Ca2+]o-induced change in [Ca2+]I. This system might provide a compensatory pathway for negative feedback regulation of parathyroid hormone secretion under physiological conditions. The Physiological Society of Japan and Springer Japan, 2013 Parathyroid gland nationallicence Calcium nationallicence Dihydropyridine receptor nationallicence Calcium receptor nationallicence Calcium channel nationallicence Iida Rinako Department of Nephrology and Hypertension, Jikei University School of Medicine, 3-25-8 Nishishinbashi, 105-8461, Minato-ku, Tokyo, Japan aut Yokoyama Keitaro Department of Nephrology and Hypertension, Jikei University School of Medicine, 3-25-8 Nishishinbashi, 105-8461, Minato-ku, Tokyo, Japan aut Ohkido Ichiro Department of Nephrology and Hypertension, Jikei University School of Medicine, 3-25-8 Nishishinbashi, 105-8461, Minato-ku, Tokyo, Japan aut Tabei Isao Department of Surgery, Jikei University School of Medicine, Tokyo, Japan aut Takeyama Hiroshi Department of Surgery, Jikei University School of Medicine, Tokyo, Japan aut Suzuki Akifumi Department of Pharmacotherapeutics, Keio University Faculty of Pharmacy, Tokyo, Japan aut Shibasaki Toshiaki Department of Pharmacotherapeutics, Keio University Faculty of Pharmacy, Tokyo, Japan aut Matsuba Douchi Department of Cell Physiology, Jikei University School of Medicine, Tokyo, Japan aut Suda Norio Department of Cell Physiology, Jikei University School of Medicine, Tokyo, Japan aut Hosoya Tatsuo Department of Nephrology and Hypertension, Jikei University School of Medicine, 3-25-8 Nishishinbashi, 105-8461, Minato-ku, Tokyo, Japan aut The Journal of Physiological Sciences Springer Japan 63/4(2013-07-01), 235-240 1880-6546 63:4<235 2013 63 12576 https://doi.org/10.1007/s12576-013-0255-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-013-0255-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Iida Rinako Department of Nephrology and Hypertension, Jikei University School of Medicine, 3-25-8 Nishishinbashi, 105-8461, Minato-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Yokoyama Keitaro Department of Nephrology and Hypertension, Jikei University School of Medicine, 3-25-8 Nishishinbashi, 105-8461, Minato-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Ohkido Ichiro Department of Nephrology and Hypertension, Jikei University School of Medicine, 3-25-8 Nishishinbashi, 105-8461, Minato-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Tabei Isao Department of Surgery, Jikei University School of Medicine, Tokyo, Japan aut NATIONALLICENCE 700 1- Takeyama Hiroshi Department of Surgery, Jikei University School of Medicine, Tokyo, Japan aut NATIONALLICENCE 700 1- Suzuki Akifumi Department of Pharmacotherapeutics, Keio University Faculty of Pharmacy, Tokyo, Japan aut NATIONALLICENCE 700 1- Shibasaki Toshiaki Department of Pharmacotherapeutics, Keio University Faculty of Pharmacy, Tokyo, Japan aut NATIONALLICENCE 700 1- Matsuba Douchi Department of Cell Physiology, Jikei University School of Medicine, Tokyo, Japan aut NATIONALLICENCE 700 1- Suda Norio Department of Cell Physiology, Jikei University School of Medicine, Tokyo, Japan aut NATIONALLICENCE 700 1- Hosoya Tatsuo Department of Nephrology and Hypertension, Jikei University School of Medicine, 3-25-8 Nishishinbashi, 105-8461, Minato-ku, Tokyo, Japan aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/4(2013-07-01), 235-240 1880-6546 63:4<235 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer