naa a22 4500 510805833 CHVBK 20180411083418.0 cr unu---uuuuu 180411e20130701xx s 000 0 eng 10.1007/s12576-013-0261-z doi (NATIONALLICENCE)springer-10.1007/s12576-013-0261-z Emulsified isoflurane postconditioning produces cardioprotection against myocardial ischemia-reperfusion injury in rats [Elektronische Daten] [Zhao-Yang Hu, Geoffrey Abbott, Ya-Dong Fang, Yue-Sheng Huang, Jin Liu] Emulsified isoflurane (EIso) preconditioning can induce cardioprotection. We investigated whether EIso application after ischemia protects hearts against reperfusion injury and whether it is mediated by the inhibition of apoptosis. Rats were subjected to 30-min coronary occlusion followed by 180-min reperfusion. At the onset of reperfusion, rats were intravenously administered saline (sham, control group), 30% intralipid (IL group) or 2mlkg−1 EIso (EIso group) for 30min. After reperfusion, infarct sizes, myocardial apoptosis and expression of Bcl-2, Bax and caspase-3 proteins were determined. Hemodynamic parameters were not different among groups. Compared with control and intralipid group, EIso limited infarct size, inhibited apoptosis, increased the expression of Bcl-2, decreased the expression of Bax, cleaved caspase-3, and enhanced Bcl-2/Bax ratio. EIso protects hearts against reperfusion injury when administered at the onset of reperfusion, which may be mediated by the inhibition of apoptosis via modulation of the expression of pro- and anti-apoptotic proteins. The Physiological Society of Japan and Springer Japan, 2013 Emulsified isoflurane nationallicence Ischemia and reperfusion injury nationallicence Cardioprotection nationallicence Hu Zhao-Yang Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut Abbott Geoffrey Department of Pharmacology, Physiology and Biophysics, School of Medicine, University of California, Irvine, Irvine, CA, USA aut Fang Ya-Dong State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China aut Huang Yue-Sheng State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China aut Liu Jin Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut The Journal of Physiological Sciences Springer Japan 63/4(2013-07-01), 251-261 1880-6546 63:4<251 2013 63 12576 https://doi.org/10.1007/s12576-013-0261-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-013-0261-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Zhao-Yang Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Abbott Geoffrey Department of Pharmacology, Physiology and Biophysics, School of Medicine, University of California, Irvine, Irvine, CA, USA aut NATIONALLICENCE 700 1- Fang Ya-Dong State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China aut NATIONALLICENCE 700 1- Huang Yue-Sheng State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China aut NATIONALLICENCE 700 1- Liu Jin Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/4(2013-07-01), 251-261 1880-6546 63:4<251 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer