naa a22 4500 510805868 CHVBK 20180411083418.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s12576-013-0276-5 doi (NATIONALLICENCE)springer-10.1007/s12576-013-0276-5 A simple machine vision-driven system for measuring optokinetic reflex in small animals [Elektronische Daten] [Yoshihiro Shirai, Kenta Asano, Yoshihiro Takegoshi, Shu Uchiyama, Yuki Nonobe, Toshihide Tabata] The optokinetic reflex (OKR) is useful to monitor the function of the visual and motor nervous systems. However, OKR measurement is not open to all because dedicated commercial equipment or detailed instructions for building in-house equipment is rarely offered. Here we describe the design of an easy-to-install/use yet reliable OKR measuring system including a computer program to visually locate the pupil and a mathematical procedure to estimate the pupil azimuth from the location data. The pupil locating program was created on a low-cost machine vision development platform, whose graphical user interface allows one to compose and operate the program without programming expertise. Our system located mouse pupils at a high success rate (~90%), estimated their azimuth precisely (~94%), and detected changes in OKR gain due to the pharmacological modulation of the cerebellar flocculi. The system would promote behavioral assessment in physiology, pharmacology, and genetics. The Physiological Society of Japan and Springer Japan, 2013 Eye movement nationallicence Visual system nationallicence Cerebellum nationallicence Mouse nationallicence OKR : Optokinetic reflex nationallicence VBAI : Vision Builder for Automated Inspection nationallicence Shirai Yoshihiro Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, 3190 Gofuku, 930-8555, Toyama, Toyama, Japan aut Asano Kenta Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, 3190 Gofuku, 930-8555, Toyama, Toyama, Japan aut Takegoshi Yoshihiro Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, 3190 Gofuku, 930-8555, Toyama, Toyama, Japan aut Uchiyama Shu Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, 3190 Gofuku, 930-8555, Toyama, Toyama, Japan aut Nonobe Yuki Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, 3190 Gofuku, 930-8555, Toyama, Toyama, Japan aut Tabata Toshihide Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, 3190 Gofuku, 930-8555, Toyama, Toyama, Japan aut The Journal of Physiological Sciences Springer Japan 63/5(2013-09-01), 395-399 1880-6546 63:5<395 2013 63 12576 https://doi.org/10.1007/s12576-013-0276-5 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-013-0276-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shirai Yoshihiro Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, 3190 Gofuku, 930-8555, Toyama, Toyama, Japan aut NATIONALLICENCE 700 1- Asano Kenta Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, 3190 Gofuku, 930-8555, Toyama, Toyama, Japan aut NATIONALLICENCE 700 1- Takegoshi Yoshihiro Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, 3190 Gofuku, 930-8555, Toyama, Toyama, Japan aut NATIONALLICENCE 700 1- Uchiyama Shu Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, 3190 Gofuku, 930-8555, Toyama, Toyama, Japan aut NATIONALLICENCE 700 1- Nonobe Yuki Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, 3190 Gofuku, 930-8555, Toyama, Toyama, Japan aut NATIONALLICENCE 700 1- Tabata Toshihide Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, 3190 Gofuku, 930-8555, Toyama, Toyama, Japan aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/5(2013-09-01), 395-399 1880-6546 63:5<395 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer