naa a22 4500 510805906 CHVBK 20180411083418.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s12576-013-0275-6 doi (NATIONALLICENCE)springer-10.1007/s12576-013-0275-6 Interleukin-1β suppresses activity of an inwardly rectifying K+ channel in human renal proximal tubule cells [Elektronische Daten] [Kazuyoshi Nakamura, You Komagiri, Manabu Kubokawa] We investigated the effect of interleukin-1β (IL-1β) on activity of an inwardly rectifying K+ channel in cultured human proximal tubule cells (RPTECs), using the patch-clamp technique and Fura-2 Ca2+ imaging. IL-1β (15pg/ml) acutely reduced K+ channel activity in cell-attached patches. This effect was blocked by the IL-1 receptor antagonist (20ng/ml), an inhibitor of phospholipase C, neomycin (300μM), and an inhibitor of protein kinase C (PKC), GF109203X (500nM). The Fura-2 Ca2+ imaging revealed that IL-1β increased intracellular Ca2+ concentration even after removal of extracellular Ca2+, which was blocked by an inhibitor of inositol 1,4,5-trisphosphate receptors, 2-aminoethoxydiphenyl borate (2-APB, 1μM). Moreover, IL-1β suppressed channel activity in the presence of 2-APB without extracellular Ca2+. These results suggest that IL-1β suppresses K+ channel activity in RPTECs through binding to its specific receptor and activation of the PKC pathway even though intracellular Ca2+ does not increase. The Physiological Society of Japan and Springer Japan, 2013 Cytokine nationallicence Patch-clamp nationallicence Fura-2 nationallicence Ca2+ nationallicence PKC nationallicence Nakamura Kazuyoshi Department of Physiology, Iwate Medical University School of Medicine, 2-1-1 Nishitokuta, 028-3694, Yahaba, Japan aut Komagiri You Department of Physiology, Iwate Medical University School of Medicine, 2-1-1 Nishitokuta, 028-3694, Yahaba, Japan aut Kubokawa Manabu Department of Physiology, Iwate Medical University School of Medicine, 2-1-1 Nishitokuta, 028-3694, Yahaba, Japan aut The Journal of Physiological Sciences Springer Japan 63/5(2013-09-01), 377-387 1880-6546 63:5<377 2013 63 12576 https://doi.org/10.1007/s12576-013-0275-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-013-0275-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakamura Kazuyoshi Department of Physiology, Iwate Medical University School of Medicine, 2-1-1 Nishitokuta, 028-3694, Yahaba, Japan aut NATIONALLICENCE 700 1- Komagiri You Department of Physiology, Iwate Medical University School of Medicine, 2-1-1 Nishitokuta, 028-3694, Yahaba, Japan aut NATIONALLICENCE 700 1- Kubokawa Manabu Department of Physiology, Iwate Medical University School of Medicine, 2-1-1 Nishitokuta, 028-3694, Yahaba, Japan aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/5(2013-09-01), 377-387 1880-6546 63:5<377 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer