naa a22 4500 510805914 CHVBK 20180411083418.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s12576-013-0270-y doi (NATIONALLICENCE)springer-10.1007/s12576-013-0270-y Lobe-related concentration- and Ca2+-dependent interactions of calmodulin with C- and N-terminal tails of the CaV1.2 channel [Elektronische Daten] [Guilin He, Feng Guo, Tong Zhu, Dongxue Shao, Rui Feng, Dandan Yin, Xuefei Sun, Huiyuan Hu, Ahhyeon Hwang, Etsuko Minobe, Masaki Kameyama, Liying Hao] This study examined the bindings of calmodulin (CaM) and its mutants with the C- and N-terminal tails of the voltage-gated Ca2+ channel CaV1.2 at different CaM and Ca2+ concentrations ([Ca2+]) by using the pull-down assay method to obtain basic information on the binding mode, including its concentration- and Ca2+-dependencies. Our data show that more than one CaM molecule could bind to the CaV1.2 C-terminal tail at high [Ca2+]. Additionally, the C-lobe of CaM is highly critical in sensing the change of [Ca2+] in its binding to the C-terminal tail of CaV1.2, and the binding between CaM and the N-terminal tail of CaV1.2 requires high [Ca2+]. Our data provide new details on the interactions between CaM and the CaV1.2 channel. The Physiological Society of Japan and Springer Japan, 2013 Calmodulin nationallicence Ca2+ nationallicence CaV1.2 nationallicence Binding nationallicence Affinity nationallicence He Guilin Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut Guo Feng Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut Zhu Tong Laboratory of Environmental Biology, Northeastern University, 110004, Shenyang, China aut Shao Dongxue Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut Feng Rui Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut Yin Dandan Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut Sun Xuefei Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut Hu Huiyuan Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut Hwang Ahhyeon Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut Minobe Etsuko Department of Physiology, Graduate School of Medical and Dental Sciences, Kagoshima University, 890-8544, Kagoshima, Japan aut Kameyama Masaki Department of Physiology, Graduate School of Medical and Dental Sciences, Kagoshima University, 890-8544, Kagoshima, Japan aut Hao Liying Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut The Journal of Physiological Sciences Springer Japan 63/5(2013-09-01), 345-353 1880-6546 63:5<345 2013 63 12576 https://doi.org/10.1007/s12576-013-0270-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-013-0270-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- He Guilin Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Guo Feng Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Zhu Tong Laboratory of Environmental Biology, Northeastern University, 110004, Shenyang, China aut NATIONALLICENCE 700 1- Shao Dongxue Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Feng Rui Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Yin Dandan Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Sun Xuefei Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Hu Huiyuan Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Hwang Ahhyeon Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Minobe Etsuko Department of Physiology, Graduate School of Medical and Dental Sciences, Kagoshima University, 890-8544, Kagoshima, Japan aut NATIONALLICENCE 700 1- Kameyama Masaki Department of Physiology, Graduate School of Medical and Dental Sciences, Kagoshima University, 890-8544, Kagoshima, Japan aut NATIONALLICENCE 700 1- Hao Liying Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/5(2013-09-01), 345-353 1880-6546 63:5<345 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer