naa a22 4500 510805922 CHVBK 20180411083418.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s12576-013-0271-x doi (NATIONALLICENCE)springer-10.1007/s12576-013-0271-x Contribution of quantitative changes in individual ionic current systems to the embryonic development of ventricular myocytes: a simulation study [Elektronische Daten] [Chikako Okubo, Hitomi Sano, Yasuhiro Naito, Masaru Tomita] Early embryonic rodent ventricular cells exhibit spontaneous action potential (AP), which disappears in later developmental stages. Here, we used 3 mathematical models—the Kyoto, Ten Tusscher-Panfilov, and Luo-Rudy models—to present an overview of the functional landscape of developmental changes in embryonic ventricular cells. We switched the relative current densities of 9 ionic components in the Kyoto model, and 160 of 512 representative combinations were predicted to result in regular spontaneous APs, in which the quantitative changes in Na+ current (I Na) and funny current (I f) made large contributions to a wide range of basic cycle lengths. In all three models, the increase in inward rectifier current (I K1) before the disappearance of I f was predicted to result in abnormally high intracellular Ca2+ concentrations. Thus, we demonstrated that the developmental changes in APs were well represented, as I Na increased before the disappearance of I f, followed by a 10-fold increase in I K1. The Author(s), 2013 Computer simulation nationallicence Ion channels nationallicence Cardiac ventricular cells nationallicence Development nationallicence Electrophysiology nationallicence Spontaneous activity nationallicence Okubo Chikako Institute for Advanced Biosciences, Keio University, 252-0882, Fujisawa, Kanagawa, Japan aut Sano Hitomi Institute for Advanced Biosciences, Keio University, 252-0882, Fujisawa, Kanagawa, Japan aut Naito Yasuhiro Institute for Advanced Biosciences, Keio University, 252-0882, Fujisawa, Kanagawa, Japan aut Tomita Masaru Institute for Advanced Biosciences, Keio University, 252-0882, Fujisawa, Kanagawa, Japan aut The Journal of Physiological Sciences Springer Japan 63/5(2013-09-01), 355-367 1880-6546 63:5<355 2013 63 12576 https://doi.org/10.1007/s12576-013-0271-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-013-0271-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Okubo Chikako Institute for Advanced Biosciences, Keio University, 252-0882, Fujisawa, Kanagawa, Japan aut NATIONALLICENCE 700 1- Sano Hitomi Institute for Advanced Biosciences, Keio University, 252-0882, Fujisawa, Kanagawa, Japan aut NATIONALLICENCE 700 1- Naito Yasuhiro Institute for Advanced Biosciences, Keio University, 252-0882, Fujisawa, Kanagawa, Japan aut NATIONALLICENCE 700 1- Tomita Masaru Institute for Advanced Biosciences, Keio University, 252-0882, Fujisawa, Kanagawa, Japan aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/5(2013-09-01), 355-367 1880-6546 63:5<355 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer