naa a22 4500 510805957 CHVBK 20180411083418.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s12576-012-0239-2 doi (NATIONALLICENCE)springer-10.1007/s12576-012-0239-2 The missing link between long-term stimulation of nicotinic receptors and the increases of acetylcholine release and vasodilation in the cerebral cortex of aged rats [Elektronische Daten] [Sae Uchida, Harumi Hotta, Hidemi Misawa, Koichiro Kawashima] In adult rats (4-9months), chronic nicotine infusion increases the basal level of acetylcholine (ACh) release in the cerebral cortex and enhances responses of cortical ACh release and cortical vasodilation elicited by nucleus basalis of Meynert (NBM) stimulation. In the present study, we examined whether these effects of nicotine are detected in aged rats. Aged rats (27-30months) received sustained subcutaneous nicotine (100μg/kg/h) or saline for 14days. Under urethane anesthesia, ACh release and regional blood flow in the parietal cortex were measured. The basal level of ACh release in the cerebral cortex was not changed by chronic nicotine. In addition, the magnitudes of ACh release and vasodilation by NBM stimulation were similar between the saline-treated and nicotine-treated groups. The lack of an effect of chronic nicotine in aged rats may be due to a decrease in nicotinic receptors in the cerebral cortex during aging (Nordberg et al., J Neurosci Res 31:103-111, 1992). The Physiological Society of Japan and Springer Japan, 2012 Chronic nicotine nationallicence Nucleus basalis of Meynert nationallicence Cortical cerebral blood flow nationallicence Acetylcholine release nationallicence Aging nationallicence Rat nationallicence Uchida Sae Department of Autonomic Neuroscience, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashi-ku, 173-0015, Tokyo, Japan aut Hotta Harumi Department of Autonomic Neuroscience, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashi-ku, 173-0015, Tokyo, Japan aut Misawa Hidemi Department of Pharmacology, Faculty of Pharmacy, Keio University, Minato-ku, Tokyo, Japan aut Kawashima Koichiro Department of Molecular Pharmacology, Kitasato University School of Pharmacy, Minato-ku, Tokyo, Japan aut The Journal of Physiological Sciences Springer Japan 63/2(2013-03-01), 95-101 1880-6546 63:2<95 2013 63 12576 https://doi.org/10.1007/s12576-012-0239-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-012-0239-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Uchida Sae Department of Autonomic Neuroscience, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashi-ku, 173-0015, Tokyo, Japan aut NATIONALLICENCE 700 1- Hotta Harumi Department of Autonomic Neuroscience, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashi-ku, 173-0015, Tokyo, Japan aut NATIONALLICENCE 700 1- Misawa Hidemi Department of Pharmacology, Faculty of Pharmacy, Keio University, Minato-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Kawashima Koichiro Department of Molecular Pharmacology, Kitasato University School of Pharmacy, Minato-ku, Tokyo, Japan aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/2(2013-03-01), 95-101 1880-6546 63:2<95 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer