naa a22 4500 510805981 CHVBK 20180411083418.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s12576-012-0250-7 doi (NATIONALLICENCE)springer-10.1007/s12576-012-0250-7 Electrophysiological and pharmacological properties of GABAergic cells in the dorsal raphe nucleus [Elektronische Daten] [Yoshihiro Gocho, Atsushi Sakai, Yuchio Yanagawa, Hidenori Suzuki, Fumihito Saitow] The dorsal raphe nucleus (DRN) is the origin of the central serotonin [5-hydroxytryptamine (5-HT)] system and plays an important role in the regulation of many physiological functions such as sleep/arousal, food intake and mood. In order to understand the regulatory mechanisms of 5-HT system, characterization of the types of neurons is necessary. We performed electrophysiological recordings in acute slices of glutamate decarboxylase 67-green fluorescent protein knock-in mice. We utilized this mouse to identify visually GABAergic cells. Especially, we examined postsynaptic responses mediated by 5-HT receptors between GABAergic and serotonergic cells in the DRN. Various current responses were elicited by 5-HT and 5-HT1A or 5-HT2A/2C receptor agonists in GABAergic cells. These results suggested that multiple 5-HT receptor subtypes overlap on GABAergic cells, and their combination might control 5-HT cells. Understanding the postsynaptic 5-HT feedback mechanisms may help to elucidate the 5-HT neurotransmitter system and develop novel therapeutic approaches. The Author(s), 2012 Dorsal raphe nationallicence GABAergic nationallicence Serotonin nationallicence Electrophysiology nationallicence Gocho Yoshihiro Department of Pharmacology, Nippon Medical School, 113-8602, Tokyo, Japan aut Sakai Atsushi Department of Pharmacology, Nippon Medical School, 113-8602, Tokyo, Japan aut Yanagawa Yuchio Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine and Japan Science and Technology Agency, CREST, 371-8511, Maebashi, Japan aut Suzuki Hidenori Department of Pharmacology, Nippon Medical School, 113-8602, Tokyo, Japan aut Saitow Fumihito Department of Pharmacology, Nippon Medical School, 113-8602, Tokyo, Japan aut The Journal of Physiological Sciences Springer Japan 63/2(2013-03-01), 147-154 1880-6546 63:2<147 2013 63 12576 https://doi.org/10.1007/s12576-012-0250-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12576-012-0250-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gocho Yoshihiro Department of Pharmacology, Nippon Medical School, 113-8602, Tokyo, Japan aut NATIONALLICENCE 700 1- Sakai Atsushi Department of Pharmacology, Nippon Medical School, 113-8602, Tokyo, Japan aut NATIONALLICENCE 700 1- Yanagawa Yuchio Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine and Japan Science and Technology Agency, CREST, 371-8511, Maebashi, Japan aut NATIONALLICENCE 700 1- Suzuki Hidenori Department of Pharmacology, Nippon Medical School, 113-8602, Tokyo, Japan aut NATIONALLICENCE 700 1- Saitow Fumihito Department of Pharmacology, Nippon Medical School, 113-8602, Tokyo, Japan aut NATIONALLICENCE 773 0- The Journal of Physiological Sciences Springer Japan 63/2(2013-03-01), 147-154 1880-6546 63:2<147 2013 63 12576 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer