naa a22 4500 510815847 CHVBK 20180411083507.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s11468-012-9385-x doi (NATIONALLICENCE)springer-10.1007/s11468-012-9385-x Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes [Elektronische Daten] [Manola Moretti, Remo Proietti Zaccaria, Emiliano Descrovi, Gobind Das, Marco Leoncini, Carlo Liberale, Francesco De Angelis, Enzo Di Fabrizio] Tip-enhanced Raman spectroscopy provides chemical information while raster scanning samples with topographical detail. The coupling of atomic force microscopy and Raman spectroscopy in top illumination optical setup is a powerful configuration to resolve nanometer structures while collecting reflection mode backscattered signal. Here, we theoretically calculate the field enhancement generated by TER spectroscopy with top illumination geometry and we apply the technique to the characterization of insulin amyloid fibrils. We experimentally confirm that this technique is able to enhance the Raman signal of the polypeptide chain by a factor of 105, thus revealing details down to few molecules resolution. The Author(s), 2012 TERS nationallicence Amyloid fibrils nationallicence Top-illumination setup nationallicence Field enhancement nationallicence Raman spectroscopy nationallicence Moretti Manola Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy aut Proietti Zaccaria Remo Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy aut Descrovi Emiliano Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy aut Das Gobind Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy aut Leoncini Marco Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy aut Liberale Carlo Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy aut De Angelis Francesco Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy aut Di Fabrizio Enzo Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy aut Plasmonics Springer US; http://www.springer-ny.com 8/1(2013-03-01), 25-33 1557-1955 8:1<25 2013 8 11468 https://doi.org/10.1007/s11468-012-9385-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11468-012-9385-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moretti Manola Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy aut NATIONALLICENCE 700 1- Proietti Zaccaria Remo Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy aut NATIONALLICENCE 700 1- Descrovi Emiliano Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy aut NATIONALLICENCE 700 1- Das Gobind Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy aut NATIONALLICENCE 700 1- Leoncini Marco Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy aut NATIONALLICENCE 700 1- Liberale Carlo Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy aut NATIONALLICENCE 700 1- De Angelis Francesco Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy aut NATIONALLICENCE 700 1- Di Fabrizio Enzo Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy aut NATIONALLICENCE 773 0- Plasmonics Springer US; http://www.springer-ny.com 8/1(2013-03-01), 25-33 1557-1955 8:1<25 2013 8 11468 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer