naa a22 4500 510815901 CHVBK 20180411083507.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s11468-012-9367-z doi (NATIONALLICENCE)springer-10.1007/s11468-012-9367-z Reading Biochips by Raman and Surface-Enhanced Raman Spectroscopies [Elektronische Daten] [Keren Kantarovich, Inbal Tsarfati-BarAd, Levi Gheber, Karsten Haupt, Ilana Bar] Biochips are a rapidly increasing research field, driven by the versatility of sensing devices and the importance of their applications. The regular approaches for creating biochips and for reading them suffer from some limitations, motivating development of miniature biochips and label-free formats. To push forward these challenges, we have chosen to combine the methods of printing of droplets of synthetic receptors by pipettes or nanofountain pens with detection by Raman spectroscopy or its surface-assisted plasmon variant, namely, surface-enhanced Raman spectroscopy (SERS). The selected receptors included molecularly imprinted polymers (MIPs), produced by polymerization of functional and cross-linking monomers around a molecular template, the β-blocking drug propranolol. The measured Raman and SERS spectra of the MIP constituents enabled identification of the template presence and consequently chemical imaging of individual and multiple dots in an array. This concept, combining nanolithography techniques with SERS paves the road toward miniaturized arrayed MIP sensors with label-free, specific and quantitative molecular recognition. Springer Science+Business Media, LLC, 2012 Raman nationallicence Surface-enhanced Raman spectroscopy nationallicence Molecularly imprinted polymers nationallicence Biochips nationallicence Nanofountain pen nationallicence Kantarovich Keren Department of Physics, Ben-Gurion University of the Negev, 84105, Beer-Sheva, Israel aut Tsarfati-BarAd Inbal Department of Biotechnology Engineering, Ben-Gurion University of the Negev, 84105, Beer-Sheva, Israel aut Gheber Levi Department of Biotechnology Engineering, Ben-Gurion University of the Negev, 84105, Beer-Sheva, Israel aut Haupt Karsten Compiègne University of Technology, UMR CNRS 6022, 60200, Compiègne, France aut Bar Ilana Department of Physics, Ben-Gurion University of the Negev, 84105, Beer-Sheva, Israel aut Plasmonics Springer US; http://www.springer-ny.com 8/1(2013-03-01), 3-12 1557-1955 8:1<3 2013 8 11468 https://doi.org/10.1007/s11468-012-9367-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11468-012-9367-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kantarovich Keren Department of Physics, Ben-Gurion University of the Negev, 84105, Beer-Sheva, Israel aut NATIONALLICENCE 700 1- Tsarfati-BarAd Inbal Department of Biotechnology Engineering, Ben-Gurion University of the Negev, 84105, Beer-Sheva, Israel aut NATIONALLICENCE 700 1- Gheber Levi Department of Biotechnology Engineering, Ben-Gurion University of the Negev, 84105, Beer-Sheva, Israel aut NATIONALLICENCE 700 1- Haupt Karsten Compiègne University of Technology, UMR CNRS 6022, 60200, Compiègne, France aut NATIONALLICENCE 700 1- Bar Ilana Department of Physics, Ben-Gurion University of the Negev, 84105, Beer-Sheva, Israel aut NATIONALLICENCE 773 0- Plasmonics Springer US; http://www.springer-ny.com 8/1(2013-03-01), 3-12 1557-1955 8:1<3 2013 8 11468 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer