naa a22 4500 510816754 CHVBK 20180411083511.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s11468-012-9457-y doi (NATIONALLICENCE)springer-10.1007/s11468-012-9457-y The Effect of Aspect Ratio of Gold Nanorods on Cell Imaging with Two-Photon Excitation [Elektronische Daten] [Xi Wu, Jing Wang, Ji-Yao Chen] To make the gold nanorod (AuNR) a better photoluminescence (PL) probe for cell imaging under two-photon excitation (TPE), the effect of the aspect ratio of AuNRs was studied. The AuNRs with the aspect ratios of 2.7, 3.2, 4.1, and 4.5 and correlated longitudinal surface plasmon resonance (LSPR) bands of 710, 760, 820, and 870nm were compared. The approach of two-photon excited PL was used to measure the two-photon absorption cross section (TPACS) of these AuNRs in aqueous solutions. Under TPE of an 800-nm femtosecond laser, the TPACS of AuNRs with an aspect ratio of 3.2 was found to be the highest (about 3 × 109GM), and that of AuNRs (aspect ratio of 2.7) was only 1.5 × 109GM. The probe function of these two AuNRs was further compared in cell imaging studies using the human liver cancer cell (QGY) as the cell model. Both TPE PL image and confocal reflectance image of AuNR-loaded cells were acquired comparatively in measurements. The brightness and contrast of confocal reflectance images for these two AuNRs in cells are similar. In contrast, the PL images of cellular AuNRs (2.7) under TPE of 800nm are weak but that of cellular AuNRs (3.2) is much better. These results show that when the LSPR band of AuNRs is coincided with the excitation wavelength, the TPACS of these AuNRs will be enhanced ensuring a good quality of cell imaging under TPE. The LSPR band is correlated to the aspect ratio of AuNRs. Therefore, in cell imaging studies with TPE, the aspect ratio effect of AuNRs should be taken into consideration. Springer Science+Business Media New York, 2012 Gold nanoparticles nationallicence Two-photon excitation absorption cross section nationallicence Two-photon photoluminescence nationallicence Cell imaging nationallicence Surface plasmon resonance nationallicence Wu Xi State Key Laboratory of Surface Physics and Department of Physics, and the Key Laboratory of Micro/Nano Photonics Structure (Ministry of Education), Fudan University, Shanghai, China aut Wang Jing State Key Laboratory of Surface Physics and Department of Physics, and the Key Laboratory of Micro/Nano Photonics Structure (Ministry of Education), Fudan University, Shanghai, China aut Chen Ji-Yao State Key Laboratory of Surface Physics and Department of Physics, and the Key Laboratory of Micro/Nano Photonics Structure (Ministry of Education), Fudan University, Shanghai, China aut Plasmonics Springer US; http://www.springer-ny.com 8/2(2013-06-01), 685-691 1557-1955 8:2<685 2013 8 11468 https://doi.org/10.1007/s11468-012-9457-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11468-012-9457-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Xi State Key Laboratory of Surface Physics and Department of Physics, and the Key Laboratory of Micro/Nano Photonics Structure (Ministry of Education), Fudan University, Shanghai, China aut NATIONALLICENCE 700 1- Wang Jing State Key Laboratory of Surface Physics and Department of Physics, and the Key Laboratory of Micro/Nano Photonics Structure (Ministry of Education), Fudan University, Shanghai, China aut NATIONALLICENCE 700 1- Chen Ji-Yao State Key Laboratory of Surface Physics and Department of Physics, and the Key Laboratory of Micro/Nano Photonics Structure (Ministry of Education), Fudan University, Shanghai, China aut NATIONALLICENCE 773 0- Plasmonics Springer US; http://www.springer-ny.com 8/2(2013-06-01), 685-691 1557-1955 8:2<685 2013 8 11468 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer