naa a22 4500 510816975 CHVBK 20180411083512.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s11468-013-9530-1 doi (NATIONALLICENCE)springer-10.1007/s11468-013-9530-1 Detect the Hybridization of Single-Stranded DNA by Parallel Scan Spectral Surface Plasmon Resonance Imaging [Elektronische Daten] [Xinyuan Chong, Le Liu, Zhiyi Liu, Suihua Ma, Jun Guo, Yanhong Ji, Yonghong He] We investigate the ability of a parallel scan spectral surface plasmon resonance imaging system in analyzing hybridization of single-stranded DNA (ssDNA) on microarray. The ssDNA probes are modified by a thiol group and thereby can be immobilized onto the gold film. Hybridization experiments are carried out by using both complementary and noncomplementary sequence to confirm the specificity of interaction. We also demonstrate that the data analysis is very important in reducing the noise and improving the resolution by comparing polynomial fitting method with the combination of polynomial fitting and centroid method. Finally, the results demonstrate that the parallel scan spectral surface plasmon resonance imaging system can be used for high-throughput analysis of the hybridization of the ssDNA. Springer Science+Business Media New York, 2013 Surface plasmon resonance nationallicence Hybridization nationallicence Microarray nationallicence Chong Xinyuan Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, 518055, Shenzhen, People's Republic of China aut Liu Le Laboratory of Advanced Power Source, Graduate School at Shenzhen, Tsinghua University, 518055, Shenzhen, People's Republic of China aut Liu Zhiyi Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, 518055, Shenzhen, People's Republic of China aut Ma Suihua Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, 518055, Shenzhen, People's Republic of China aut Guo Jun Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, 518055, Shenzhen, People's Republic of China aut Ji Yanhong MOE Key laboratory of Laser Life Science & Institute of Laser Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut He Yonghong Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, 518055, Shenzhen, People's Republic of China aut Plasmonics Springer US; http://www.springer-ny.com 8/2(2013-06-01), 1185-1191 1557-1955 8:2<1185 2013 8 11468 https://doi.org/10.1007/s11468-013-9530-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11468-013-9530-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chong Xinyuan Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, 518055, Shenzhen, People's Republic of China aut NATIONALLICENCE 700 1- Liu Le Laboratory of Advanced Power Source, Graduate School at Shenzhen, Tsinghua University, 518055, Shenzhen, People's Republic of China aut NATIONALLICENCE 700 1- Liu Zhiyi Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, 518055, Shenzhen, People's Republic of China aut NATIONALLICENCE 700 1- Ma Suihua Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, 518055, Shenzhen, People's Republic of China aut NATIONALLICENCE 700 1- Guo Jun Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, 518055, Shenzhen, People's Republic of China aut NATIONALLICENCE 700 1- Ji Yanhong MOE Key laboratory of Laser Life Science & Institute of Laser Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- He Yonghong Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, 518055, Shenzhen, People's Republic of China aut NATIONALLICENCE 773 0- Plasmonics Springer US; http://www.springer-ny.com 8/2(2013-06-01), 1185-1191 1557-1955 8:2<1185 2013 8 11468 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer