naa a22 4500 510817092 CHVBK 20180411083512.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s11468-012-9476-8 doi (NATIONALLICENCE)springer-10.1007/s11468-012-9476-8 A Label-Free Nanogold DNAzyme-Cleaved Surface-Enhanced Resonance Raman Scattering Method for Trace UO2 2+ Using Rhodamine 6G as Probe [Elektronische Daten] [Zhiliang Jiang, Dongmei Yao, Guiqing Wen, Tingsheng Li, Bin Chen, Aihui Liang] In pH 5.5 2-(N-morpholino)-ethanosulfonic acid buffer solution containing 0.25M NaCl at 80°C, the single-stranded substrate DNA hybridizes with the enzyme DNA to form double-stranded DNA (dsDNA). The substrate chain of dsDNA could be cracked catalytically by UO2 2+ to produce a short single-stranded DNA (ssDNA) that adsorbed on the nanogold (NG) surface to form a stable nanogold-ssDNA conjugate and then further combine with rhodamine 6G (RhG) to form a NG-ssDNA-RhG conjugate that can be monitored by the surface-enhanced resonance Raman scattering (SERRS) spectral technique at 1,360cm−1. Under the selected conditions, the increased SERRS intensity ΔI 1360 was linear to UO2 2+ concentration in the range of 5-125nmol/L, with a detection limit of 1.6nmol/L. Using a 0.5-μmol/L Hg2+ as enhancer, a 2.5-100-nmol/L UO2 2+ can be determined. Springer Science+Business Media New York, 2012 UO2 2+ nationallicence DNAzyme nationallicence Nanogold nationallicence Rhodamine 6G nationallicence Surface-enhanced resonance Raman scattering nationallicence Jiang Zhiliang Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Normal University, 541004, Guilin, China aut Yao Dongmei Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Normal University, 541004, Guilin, China aut Wen Guiqing Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Normal University, 541004, Guilin, China aut Li Tingsheng Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Normal University, 541004, Guilin, China aut Chen Bin Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Normal University, 541004, Guilin, China aut Liang Aihui Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Normal University, 541004, Guilin, China aut Plasmonics Springer US; http://www.springer-ny.com 8/2(2013-06-01), 803-810 1557-1955 8:2<803 2013 8 11468 https://doi.org/10.1007/s11468-012-9476-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11468-012-9476-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jiang Zhiliang Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Normal University, 541004, Guilin, China aut NATIONALLICENCE 700 1- Yao Dongmei Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Normal University, 541004, Guilin, China aut NATIONALLICENCE 700 1- Wen Guiqing Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Normal University, 541004, Guilin, China aut NATIONALLICENCE 700 1- Li Tingsheng Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Normal University, 541004, Guilin, China aut NATIONALLICENCE 700 1- Chen Bin Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Normal University, 541004, Guilin, China aut NATIONALLICENCE 700 1- Liang Aihui Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Normal University, 541004, Guilin, China aut NATIONALLICENCE 773 0- Plasmonics Springer US; http://www.springer-ny.com 8/2(2013-06-01), 803-810 1557-1955 8:2<803 2013 8 11468 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer