naa a22 4500 510817165 CHVBK 20180411083513.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s11468-012-9442-5 doi (NATIONALLICENCE)springer-10.1007/s11468-012-9442-5 Detection of Vascular Endothelial Growth Factor Based on Gold Nanoparticles and Immunoreaction Using Resonance Light Scattering [Elektronische Daten] [Zhanguang Chen, Yulong Lei, Wenhua Gao, Jinbin Liu] In the study, a new assay of vascular endothelial growth factor (VEGF) has been developed by the use of gold nanoparticles (GNPs)-anti-VEGF conjugates. The immunoreaction between GNPs-anti-VEGF conjugates and VEGF took place in pH 7.5PBS buffer solution after the addition of VEGF. The formation of GNPs modified VEGF immunocomplex resulted in the enhanced resonance light scattering (RLS) intensity at 388.0nm. Under the optimal conditions, the magnitude of enhanced RLS intensity (ΔI RLS) was proportional to the VEGF concentration in the range from 100 to 1,500pg mL−1, with a detection limit of 60pg mL−1. The surface plasma resonance absorption spectrum, the characteristics of RLS, the VEGF immunocomplex, and the optimum conditions of the immunoreaction have all been investigated. The VEGF concentrations of 20 serum specimens detected by the developed assay showed consistent results in comparison with those obtained by commercially available enzyme-linked immunosorbent assay kit. Springer Science+Business Media, LLC, 2012 Vascular endothelial growth factor nationallicence Resonance light scattering nationallicence Gold nanoparticles nationallicence Immunoreaction nationallicence Chen Zhanguang Department of Chemistry, Shantou University, 515063, Shantou, China aut Lei Yulong Department of Chemistry, Shantou University, 515063, Shantou, China aut Gao Wenhua Department of Chemistry, Shantou University, 515063, Shantou, China aut Liu Jinbin Department of Chemistry, University of Texas at Dallas, 75080, Richardson, TX, USA aut Plasmonics Springer US; http://www.springer-ny.com 8/2(2013-06-01), 605-611 1557-1955 8:2<605 2013 8 11468 https://doi.org/10.1007/s11468-012-9442-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11468-012-9442-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Zhanguang Department of Chemistry, Shantou University, 515063, Shantou, China aut NATIONALLICENCE 700 1- Lei Yulong Department of Chemistry, Shantou University, 515063, Shantou, China aut NATIONALLICENCE 700 1- Gao Wenhua Department of Chemistry, Shantou University, 515063, Shantou, China aut NATIONALLICENCE 700 1- Liu Jinbin Department of Chemistry, University of Texas at Dallas, 75080, Richardson, TX, USA aut NATIONALLICENCE 773 0- Plasmonics Springer US; http://www.springer-ny.com 8/2(2013-06-01), 605-611 1557-1955 8:2<605 2013 8 11468 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer